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银杏叶提取物对培养的人脐静脉内皮细胞结构型一氧化氮合酶的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第3期
页码:
214-215
栏目:
论著
出版日期:
2001-05-01

文章信息/Info

Title:
The Gingko biloba extract on the expression of constitutive nitric oxide synthase in human umbilical vein endothelial cells
作者:
张金霞1;  胡 涛1;  田 琼2;  李兰荪1;  贾国良1;  赵新国1
第四军医大学:1.西京医院心内科, 2.教学实验中心,陕西西安710032
Author(s):
ZHANG Jin-xia1;  HU Tao1;  TIAN Qiong2;  LI Lan-sun1;  JIA Guo-liang1;  ZHAO Xin-guo1
1.Department of Cardiovasology, Xijing Hospital, Fourth Military Medical University, Xi' an Shaanxi 710032, China
关键词:
银杏叶提取物; 人脐静脉内皮细胞; 一氧化氮合酶; 冠状动脉再狭窄
Keywords:
gingko biloba extract;  human umbilical vein endothelial cells; nitric oxide synthase;  coronary artery restenosis
分类号:
-
DOI:
-
文献标识码:
A
摘要:
目的 观察银杏叶提取物(EGB)对培养的人脐静脉内皮细胞结构型一氧化氮合酶(ecNOS)活性的影响,并探讨EGB防治PTCA后再狭窄的意义。方法 分别用10,50,100 g·L-1的EGB作用于体外培养人脐静脉内皮细胞(HUVEC),行ABC免疫酶染色,图像分析法观察EGB对NOS表达的影响。结果 与对照组比较,各实验组ecNOS表达增强(P<0.05)。结论 EGB增强了培养的内皮细胞的cNOS表达。
Abstract:
AIM To observe the effect of the gingko biloba extract (EGB) on the expression of constitutive nitric oxide synthase (ecNOS) in human umbilical vein endothelial cells(HUVEC) and to evaluate EGB can be used in the prevention of the coronary artery restenosis after percutanuous transluminal coronary angioplasty (PTCA).METHODS ABC immunohistochemistry technique and image analyzing method were used to evaluate the effect of the EGB on the expression of cNOS in cultured HUVEC. RESULT The expression of cNOS was significantly increased by EGB(P<0.05). CONCLUSION EGB can enhance the expression of cNOS in cultured HUVEC.

参考文献/References

[1] Meurice T, Vallet B, Bauters C. Role of endothelial cells in restenosis after coronary angioplasty[J]. Fundam Clin Pharmacol, 1996,10(3):234-242.

[2] Clostre F. Ginkgo biloba extract (EGb 761). State of knowledge in the dawn of the year 2000[J].Ann Pharm Fr, 1999,57 (Suppl 1):1S8-88.

[3]董为人,李 进,戴 云,等.一氧化氮抑制血管损伤引起的新生内膜形成[J].第一军医大学学报,1998,18:78-80.

[4] Oemar BS, Tschudi MR, Godoy N, et al. Reduced endothelial nitric oxide synthase expression and production in human atherosclerosclerosis[J]. Circulation,1998,97:2494-2498.

[5] Cheung F, Siow YL, Chen WZ. Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells [J].Biochem Pharmacol, 1999, 58(10):1665-1673.

[6] Hitzenberger G. The effect of ginkgo biloba special extract[J].Wien Med Wochenschr, 1992,142(17):371-379.

备注/Memo

备注/Memo:
收稿日期：2000-12-15.
更新日期/Last Update: 2001-05-01