平滑肌细胞与基质在静脉桥内膜增生的时空变化(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2001年第4期
- 页码:
- 281-284
- 栏目:
- 论著
- 出版日期:
- 2001-07-01
文章信息/Info
- Title:
- Time-course and distribution of smooth muscle cells proliferation and extracellular matrix accumulation during intimal thickening in rabbit vein grafts
- Author(s):
- ZHANG Wei-da1; ZHU Hai-long1; BAI Hong-zhi2; Tomoyuki Yamakawa2; Hikaru Matsuda2
- 1.Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University,2.First Department of Osaka University Medical School, Japan
- Keywords:
- smooth muscle cells; vein graft; extracellular matrix
- 分类号:
- R654. 2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 研究移植静脉桥内膜增厚过程中平滑肌细胞(SMC) 增殖、表型转化和细胞外基质积聚的时间变化与空间分布。方法 将兔自体颈浅静脉桥移植到颈动脉, 在术后3, 8, 15d 和2, 6 个月取血管桥行免疫组化染色。结果静脉桥中层SMC 在术后3d 表现受损并开始增殖, 8d 迁移到内层并形成新内膜层, SMC 表型从正常成熟型向胚胎型转化。术后15d, SMC 增殖率达高峰, 2月后迅速下降, 且绝大部分细胞从胚胎型转变为成熟表型, 但在术后6 月, 仍有少量胚胎型SMC 存在。新内膜层自形成后在整个观察期呈进行性增厚。内膜层细胞外基质主要成分:I 型胶原、硫酸肝素、硫酸皮肤素。术后2 月基质大多堆积在内膜深层细胞稀疏区。结论 静脉桥新内膜的形成起源于表型转化胚胎型SMC 的迁移, 细胞增殖和基质积聚使新内膜增厚。而胚胎型SMC 在新生内膜的持续存在, 可能促使基质不断堆积和新内膜层进行性增厚, 易使移植静脉桥造成狭窄。
- Abstract:
- AIM To observe the time-course proliferation and phenotypic modulation of smooth muscle cells (SMC) , with accumulation and distribution of various extracellular matrix (ECM)components during neointima formation in rabbit vein grafts. METHODS An autologous external jugular vein graft was transplanted into the carotid artery in 25 rabbits. The vein grafts were harvested at days 3, 8, 15 and 2, 6 months after operation and were stained with immunohistochemistry. RESULTS Medial SMCs in the graft appeared to be injured, and theybegan to proliferate at day 3 and subsequently migrated and formed the neointima at day 8. The neointima observed at days 8 and 15 contained ECM components, including type I collagen,heparan sulfate, and dermatan sulfate, and the intimal SMCs were pheno typically modultated from the adult-type to the embryonic-type. The intimal SMC proliferation was maximal at day 15 and then decreased rapidly. However, the neointima continued to thicken thereafter throughout the 6-month period of the experiment, and ECM accumulation was a prominent feature observed in the hypocellular region of the deep intima from 2 months after the transplantation. The pheno type of intimal SMCs gradually returned to the adult-type, but a small number of the intimal SMCs remained in the embryonic phenotype even at 6 months after the operation.CONCLUSION The neointima in the vein graft was formed initially by means of migration and proliferation of the phenotypically modulated, embryonic-type SMCs and continued to thicken by mean of sustained ECM accumulation may contribute to the frequent occurrence of graft stenosis that occurs in the vein grafts.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2001-02-17.
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