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大鼠心脏房室束各部浦氏细胞的比较(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第4期
页码:
310-312
栏目:
论著
出版日期:
2001-07-01

文章信息/Info

Title:
Comparative study for purkinje cells in different parts of the atrioventricular bundle(AVB)of rat heart
作者:
吴宝金; 朱永泽; 吴洪海
扬州大学医学院解剖学教研室，江苏扬州225001
Author(s):
WU Bao-jin; ZHU Yong-ze; WU Hong-hai
Department of Anatomy，Medica1 College of Yangzhou University，Yangzhou Jiangsu 225001,China
关键词:
房室束; 浦氏细胞; 细胞学; 体枧学; 大鼠
Keywords:
atrioventricular bundle(AVB); Purkinie cells; cytology; stereology; rat
分类号:
Q954.663
DOI:
-
文献标识码:
A
摘要:
目的 探讨大鼠心脏房室束(AVB)各部浦氏细胞的细胞学和体视学特征。方法 SD大鼠10只，切取房室交界区组织，常规处理后，Masson三色或HE染色，计算机图象分析仪测量AVB各部浦氏细胞的细胞学及体视学参数。结果 ①AVB主要由两种细胞组成，浦氏细胞，T细胞。AVB后端与房室结移行处，浦氏细胞与P细胞的形态无截然分界。②AVB由后向前，浦氏细胞浆面积增加，核浆比减小，肌原纤维增多。③AVB由后向前，浦氏细胞体积密度，表面积密度增大，而数密度不变。结论 ①AvB的浦氏细胞在AVB的不同部位处于不同的分化状态；由后向前分化逐渐成熟。 ④AVB由后向前，浦氏细胞增大导致体积密度、表面积密度增大。
Abstract:
AIM To study the cytology and stereology features of Purkinje cells in the AVB of rat．METIIODS The tissues containing AVB of rats were dehydrated in series concentration mixture of alcoho1 and norma1 butyl alcohol，embedded in paraffin and cut serlally． After stained with Masson trichroism or HE，sections were observed under the microscope． The cytological and stereological parameters were measured by the image analysis system．RESUTLS ①The AVB was composed of mairly two types cells，Purkinje ceils and T cells．There was no distinct difference between the P cells and the Purkinje cells between the atr oventricular node and the AVB．② From the posterior part to the anterior part of the AVB，the nucleocytoplasmic ratio of the Purkinje cells gradually decreased，but their plasmo-dimension and myolilaments gradually increased．③From the posterior part to the anterior part of the AVB，the volume density，the surface density of the Purkinje cells gradually increased，but the numerical density remained the same．CONCLUSION ①From the posterior part to the anterior part of AVB，the Purkinje cells developed．②From the posterior part to the anterior part of AVB，the volume density and the surface density of the Purkinje cells increased with the increase in their volume．

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-09-06.基金项目：江苏省教育厅自然科学基金(No. JW970033)
更新日期/Last Update: 2001-07-01