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氧化应激对培养心肌细胞线粒体酶活性的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第5期
页码:
346-347,351
栏目:
论著
出版日期:
2001-09-01

文章信息/Info

Title:
Effect of oxidative stress on mitochondrial enzyme activity in cultured cardiac myocytes
作者:
郑延松1;  李　源1;  臧益民2;  龚卫琴1;  孙　斌1
第四军医大学: 1. 西京医院老年病科; 2. 基础部生理学教研室, 陕西西安710032
Author(s):
ZHENG Yan-song1;  LI Yuan1;  ZANG Yi-min2;  GONG Wei-qin1;  SUN Bin1
1.Department of Geriatrics, Xijing Hospital, 2.Department of physiology, Fourth Military Medical University, Xi’an Shaanxi 710032, China
关键词:
应激;  氧化;  心肌细胞;  褪黑素;  线粒体
Keywords:
stress;  oxidative;  cardiomyocyte;  mitochondria;  melatonin
分类号:
R331.36
DOI:
-
文献标识码:
A
摘要:
目的 观察氧化应激对心肌细胞线粒体酶活性的影响和褪黑素的保护作用。方法 胰酶消化法分离培养原代心肌细胞, 随机分为: H2O2 处理组(100 Lmol/L ) 和褪黑素(MT) 预处理组(100 Lmol/L MT + 100 Lmol/L H2O2)。用H2O2 模拟心肌细胞的氧化应激, 用四唑盐比色试验(MTT 法) 检测H2O2 处理后不同时间点的线粒体酶活性; 用 台盼蓝排斥试验检测处理前和处理后40 min 细胞的存活率。结果 H2O2 处理组光密度(OD490) 逐渐降低, 组内不同时间点差异显著(P < 0. 01) ; 而MT 预处理组组内无差异(P > 0. 05) ; 40min 内两组细胞存活率均无变化。结论 低浓度的H2O2 随着时间的延长可以使线粒体酶活性逐渐降低, 而褪黑素能保护氧化应激中的心肌细胞。
Abstract:
AIM To investigate the effects of H2O2 and melaton in (MT ) on mitochondrial enzyme activity in cultured cardiac myocytes. METHODS Ventricular myocytes were isolated from neonatal mice and cultured for 3～ 4 d. Cells were divided into 2 groups randomly: H2O2 (100Lmol/L ) group and MT preconditioning group (100 Lmol/L MT + 100 Lmol/L H2O2). After cells were treated, the MTT assay was used to measure the activity of mitochondrial enzyme in cardiomyocytes. The trypan blue exclusion was used to detected the cell viability. RESULTS The OD490 values were gradually decreased in H2O2 group (P < 0. 01) , while there was no change in MT preconditioning group. The cell death was not increased in 40 minutes both in H2O2 group and in MT preconditioning group. CONCLUSION The low concentration of H2O2 can inhibit the activity of mitochondrial enzyme and melatonon can protect cardiomyocytes from the injury of H2O2 -induced oxidative stress.

参考文献/References

[1] Wu ML , Tsai KL , Wang SM , et al. Mechanism of hydrogen peroxide and hydroxyl free radical-induced intracellular acidification in cultured cardiac myoblasts [J]. Circ Res, 1996,78: 564.

[2] Harsdorf RV , Li PF, Dietz R. Signaling pathways in reactive oxygen species-induced cardiomyocyte apoptosis [J].Circulation, 1999, 99: 2934.

[3] 郑延松, 李　源, 臧益民, 等. 褪黑素对心肌细胞抗过氧化氢损伤的实验研究[J]. 心脏杂志, 2001, 13 (1) : 37.

[4] Sargent JM , Taylor CG. Appraisal of the MTT assay as a rapid test of chemosensitivity in accute myeloid leukaemia [J]. Br J Cancer, 1989, 60: 206.

[5] Scalbert E, Guardiola-Lemaitre B, Delagrange P. Melatonin and regulation of the cardiovascular system [J]. Therapie,1998, 53 (5) : 459.

备注/Memo

备注/Memo:
收稿日期：2000-06-21.
更新日期/Last Update: 2001-09-01