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钙激活性钾通道是人小肠上皮细胞的容积调节性通道(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第2期
页码:
100-105
栏目:
实验研究
出版日期:
2002-03-01

文章信息/Info

Title:
Ca2+ -activated K+ channel is avolume regulatory channel inepithel cells
作者:
王　军1;  臧益民1;  徐洪涛2
1.第四军医大学生理学教研室, 陕西 西安 710032; 2.海军总医院心血管内科
Author(s):
WANG Jun1;  ZANG Yi-min1;  XU Hong-tao2
1.Department of Physiology, Fourth Military Medical University, Xi’an , ShaanXi 710032, China 2. Department of Cardiovascular Disease,Beijing Navy Hospital
关键词:
全细胞记录;  单通道记录;  钙激活性钾通道;  调节性容积减小(RVD)
Keywords:
human epithelial intestine 407 cells;  patch clamp;  regulatory volume decrease;  Ca2+-act ivated K+ channel
分类号:
-
DOI:
-
文献标识码:
A
摘要:
目的 研究细胞容积调节机制, 探讨人类小肠上皮细胞调节性容积减小(RVD) 过程中离子通道的作用及其种类。方法 膜片钳全细胞记录和单通道记录法记录培养的人类小肠上皮细胞RVD过程中电流的变化, 细胞容积测定法观察RVD过程中特异性钙激活性钾通道阻断剂(clotrimazole)的作用。结果 全细胞记录法证实细胞RVD过程中K+ 通道和Cl2 通道电流同时被激活, 该K+ 通道电流具有明显的钙依赖性并可被clotrimazole 阻断。单通道记录法进一步证实RVD 过程中激活的K+ 通道为Intermediate-conductance 钙激活性钾通道。结论 人类小肠上皮细胞在低渗溶液作用下具有RVD过程, 钙激活性钾通道在RVD过程中具有十分重要的作用。
Abstract:
The regulatory volume decrease (RVD) is an essential function of the cell. In most cell types, RVD is accomplished mainly by KCl efflux induced by parallel activation of K + and Cl- channels. This investigation was aimed to study the K + channel that was responsible for the RVD in human intestine 407 cells using patch clamp. Whole-cell recordings showed that hypotonic stress could activate K + current which depended on the intracellular Ca 2+ and was blocked reversibly by an specific intermediate-conductance K + channel(IK) blocker, clotrimazole. This channel was also activated by hypotonic challenge in cell-attached mode. Cell volume measurement confirmed the electrophysiological results. Thus, it is concluded that the IK channel is responsible for K + efflux during the RVD in Intestine 407 cells.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2001-08-28.
更新日期/Last Update: 2002-03-01