我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

不同强度恒磁场对人脐动脉平滑肌细胞增殖的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第2期
页码:
113-115
栏目:
实验研究
出版日期:
2002-03-01

文章信息/Info

Title:
Effects of constant magnetic fields of different intensities on the proliferation of human umbilical arterial vascular smooth muscle cells
作者:
冯旭阳;  贾国良;  赵新国;  张荣庆;  李　妍;  曹　丰;  王海昌;  吕安林
第四军医大学西京医院心血管内科, 陕西 西安 710032
Author(s):
FENG Xu-yang ;  JIA Guo-lang;  ZHAO Xin-guo;  ZHANG Rong-qing ;  LI Yan;  CAO Feng ; WANG Hai-chang ;  LU An-lin
Depatment of Cardiology, Xijing Hospital of Fourth Military Medical University, Xi’an Shaanxi 710032, China
关键词:
恒磁场;  血管平滑肌细胞;  人;  增殖;  强度
Keywords:
constant magnetic field;  vascular smooth muscle cells;  human;  proliferation;  intensty
分类号:
R454.1　
DOI:
-
文献标识码:
A
摘要:
目的 探讨不同强度恒磁场对离体人脐动脉平滑肌细胞 (VSMC)增殖的影响。方法 用含 10 0 ml· L- 1 新生牛血清及成纤维生长因子 (5μg· L- 1 )和表皮生长因子 (10μg· L- 1 )的 DMEM的培养液 ,培养人 VSMC至 3～ 10代 ,将培养液与 VSMC的混悬液 (细胞浓度为 1× 10 5· m L- 1 ) 10 0μL加入 96孔培养板中培养 ,对照组持续培养 72h,实验组 2 4h后将培养板置于 0 .5～ 6 0 m T的恒磁场中继续培养 48h,用 MTT法和 3 H- Td R掺入法检测两组VSMC的增殖数量 (A值和核素闪烁计数值 )。结果 在恒磁场的作用下 ,0 .5 ,1,2 ,5 m T组人脐 VSMC的增殖数量显著低于对照组 (P<0 .0 5 )。 10 ,30 ,6 0 m T组人脐 VSMC的增殖数量显著高于对照组 (P<0 .0 1)。结论 恒磁场强度为 0 .5 ,1,2 ,5 m T时抑制 VSMC增殖 ,10 ,30 ,6 0 m T时促进 VSMC增殖。
Abstract:
AIM To explore the effects of constant magnetic fields(CMF) of different intensities on the proliferation of human umbilical arterial vascular smooth muscle cells(VSMC). METHODS The VSMCs of human umbilical arterial were cultured with DMEM containing 100 mL·L -1 neonatal calf serum and fibroblast growth factor(5 μg·L -1 ), epithelial growth factor (10 μg·L -1 ) for 3～10 posterities. 100 μL mixture of DMEM and VSMC(cell density 1×10 5·mL -1 ) were put into the culture plate with 96 holes and incubated for 72 h in the control group which was not under a CMF. The experimental group was first incubated for 24 h, then CMFs with 0.5,1,2,5,10,30,60 mT was put into the culture plate for 48 h. MTT and 3H-TdR incorporation methods (A-value and cpm-value) were used to determine the amount of VSMC proliferation. RESULT The amount of VSMC proliferation was significantly lower than those of the control group under the CMFs 0.5,1,2,5 mT(P<0.05 ),but was significantly higher than those of the control group under the CMFs 10,30,60 mT (P<0.01 ). CONCLUSION The CMFs of 0.5,1,2,5 mT can significantly inhibit human VSMC proliferation and CMFs 10,30,60 mT can enhance VSMC proliferation.

参考文献/References

[1] 吕安林, 高　歌, 贾国良, 等. 恒磁场对离体兔动脉平滑肌细胞的抑制效应[J]. 中华理疗杂志, 2000, 23 (5) : 288- 290.

[2] 陈树德, 张红锋, 陈家森, 等. 低频电磁场对细胞生物学效应的影响[J]. 中华物理医学杂志, 1998, 20: 78- 80.

[3] Raylman RR, Clavo AC, Wahl RL. Exposure to strong static magnetic field slows the growth of human cancer cells in vitro[J]. Bioelectrom agnetics, 1996, 17 (5) : 358- 363.

[4] Tuinstra R, Goodman E, Greenebaum B. Protein kinase C activity following exposure to magnetic field and phorbol ester[J].Bioelectrom agnetics, 1998, 19 (8) : 469- 476.

[5] Nossol B,Buse G, Silny J. Influence weak static 50 Hzmagnetic fields on the redox activity of cytochrome C [J]. Bioelectromagnetics, 1993, 14: 361- 372.

[6] Lai H, Singh NP. Acute exposure to a 60 Hz magnetic field increases DNA strand breaks in rat brain cells [J]. Bioelectromagnetics, 1997, 18: 156- 165.

[7] 吕安林, 高　歌, 贾国良, 等. 磁场防治心血管疾病的研究进展[J]. 中华理疗杂志, 2001, 24 (1) : 58- 60.

备注/Memo

备注/Memo:
收稿日期：2001-05-18.
更新日期/Last Update: 2002-03-01