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氯沙坦对家兔早期动脉粥样硬化血管MCP-1表达及胆固醇含量的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第2期
页码:
116-118
栏目:
实验研究
出版日期:
2002-03-01

文章信息/Info

Title:
Effects of losartan on expression of monocyte chemoattractant protein-1 and aortic cholesterol content in a rabbit model of early accelerated atherosclerosis
作者:
李爱民;  何作云;  周小波;  覃　军;  耿建萌
第三军医大学新桥医院心内科, 重庆 400037
Author(s):
LI Ai-min;  HE Zuo-yun;  ZHOU Xiao-bo; QIN Jun;  GENG Jian-meng
Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
动脉粥样硬化;  单核细胞趋化蛋白-1;  血管紧张素II受体拮抗剂
Keywords:
atherosclerosis;  monocyte chemoattractant protein-1;  angiotensinⅡ receptor antagonist
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的 氯沙坦对加速性家兔早期动脉粥样硬化（AS）血管增生及MCP－1蛋白表达的影响。方法 采用组织学，免疫组织化学和生物化学方法评价加速性家兔AS早期血管组织学，增殖细胞核抗原（PCNA）及单核白细胞趋化蛋白－1（MCP－1）蛋白表达和组织胆固醇含量的改变。结果 AS组血管内膜与中膜厚度比值，主动脉组织胆固醇含量，内膜PCNA阳性细胞数及MCP－1蛋白表达平均光密度积分值均显著增加，氯沙坦干预组内膜与中膜厚度比值较AS组明显下降，PCNA阳性细胞数明显减少，血管壁MCP－1蛋白表达水平明显下降（均P＜0．01）。氯沙坦干预组主动脉组织胆固醇含量明显低于AS组[1.47±0.03mg/g(wet)与1.35±0.02mg/g(wet),P<0.05]。结论 氯沙坦干预可抑制MCP－1蛋白表达，减轻加速性AS病变血管内膜增生，减少胆固醇在血管壁沉积，从而可能在防治AS过程中起重要作用。
Abstract:
AIM To investigate effects of losartan on aortic cholesterol content and expression of monocyte chemoat tractant protein-1 (MCP-1) in a rabbit model of early accelerated atherosclerosis. METHODS Aortic cholesterol content, expression of proliferation cell nuclear antigen (PCNA ) and MCP-1 protein were observed by enzymatic analysis and immunohistochemistry. RESULTS The ratio of vascular intimal to medial thickness, aortic cholesterol content, numbers of positive PCNA cells, and mean Avalues of expression of MCP-1 protein in AS group were singnificantly increased than in control group , losartan-pretreated rabbits displayed a significant decrease of ratio of vascular intimal to medial thickness, numbers of positive PCNA cells and mean A values of expression of MCP-1 protein (P < 0. 01). Aortic cholesterol content in losartanp ret reated group were significantly decreased than that in AS group (P < 0. 05). CONCLUSION Losartan inhibits vascular MCP-1 expression, decreases cholesterol deposit in vascular walls and inhibits intimal proliferation,which may play an important role in prevention of atherosclerosis.

参考文献/References

[1] Reape TJ , Groot PH. Chemokines and atherosclerosis [J].Atherosclerosis, 1999, 147: 213- 225.

[2] Klouche M , Rose-John S, Schemidt W , et al. Enzymatically degraded nonoxidized LDL induces human vascular smooth muscle cell activation , foam cell transformation, and proliferation[J]. Circulation, 2000, 101: 1799- 1805.

[3] Hernandez-Presa M , Bustos C, Ortego M , et al. Angiotensin-converting enzyme inhibition prevents arterial nuclear factor-κ B activation, monocyte chemoattractant protein-1 expression,and macrophage infiltration in a rabbit model of early accelerated atherosclerosis[J]. Circulation, 1997, 95: 1532- 1541.

[4] Virone-Oddos A , Desangle V , Provost D, et al. In vitro and in vivo effects of UP269- 6, a new potent orally active nonpeptide angiotensin II receptor antagonist, on vascular smooth muscle cell proliferation[J]. Br J Pharmacol, 1997, 120: 488- 494.

[5] Yla-Herttualla S,Lipton BA , Rosenfeld ME, et al. Expression of monocyte chemoattractant protein-1 in macrophage-rich areas of human and rabbit atherosclerotic lesions [J]. Proc Natl Acad Sci USA , 1991, 88: 5252- 5256.

[6] Cushing SD,Berliner JA ,Valente AJ , et al. Minimally modified low density lopoprotein induced monocyte chemotactic protein-1 in human endothelial cells and smooth muscle cells[J]. Proc Natl Acad Sci USA , 1990, 87: 5134- 5138.

[7] Takai S, Shiota N , Kobayashi S, et al. Induction of chymase that forms angiotensin II in the monkey atherosclerotic aorta [J].FEBS Lett, 1997, 412: 86- 90.

备注/Memo

备注/Memo:
收稿日期: 2000-11-06.
更新日期/Last Update: 2002-03-01