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乙酰胆碱诱发犬急性心房颤动模型的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第3期
页码:
195-198
栏目:
实验研究
出版日期:
2002-05-01

文章信息/Info

Title:
The invesigate of a canine acute atrial fibrillation model induced by acetylcholine
作者:
杨诏旭1;  蔡振杰1;  王跃民2;  张晓东2;  周更须1
第四军医大学: 1. 西京医院心脏外科, 2. 基础部生理学教研室, 陕西 西安 710032
Author(s):
YANG Zhao-xu;  CAI Zhen-jie; WANG Yue-min;  ZHANG Xiao-dong; ZHOU Geng-xu
Center of Cardiovascular Disease, Xijing Hospital, Fourth Millitary Medical University, Xi’an,Shaanxi 710032, China
关键词:
乙酰胆碱;  心房快速起搏;  心房颤动;  动物模型;  狗
Keywords:
acetylcholine;  atrial burst-pacing;  atrial fibrillation;  animal model;  dogs
分类号:
R541.75
DOI:
-
文献标识码:
A
摘要:
目的 研究乙酰胆碱(ACh)诱发犬急性心房颤动模型的效果及机制。方法 取10条健康杂种犬,给予持续静注ACh及心房快速起搏,测定房颤持续时间,根据房颤持续时间逐渐增加药量,直至通过心房快速起搏可以诱发出持续性房颤(≥3 min)为止。给药前及给药后测定心率,校正窦房结恢复时间,心房有效不应期,给药前后及房颤发生后测定左室内压及左室内压最大上升、下降速率(±dp/dtmax)。结果 在给予ACh前,通过心房快速起搏均未能诱发出持续性房颤,随着ACh量的逐渐增加,诱发出房颤的时间逐渐延长,当增加到一定量(18±8μg·kg-1·min-1)之后,10只犬均可以通过心房快速起搏诱发出持续性房颤,有1只犬不需诱发即可出现,其持续时间为344±173 s。测定犬心率明显减慢(157±25vs138±20·min-1,P<0.01),校正窦房结恢复时间延长(44±13vs81±24 ms,P<0.01),心房有效不应期明显缩短(80±16vs60±13 ms,P<0.01),使用ACh后左室内压及其最大上升、下降速率差异不显著(140±29vs129±15 mm Hg·s-1,P>0.05;2752±876vs2434±531 mm Hg·s-1; -3115±1067vs-2707±714 mm Hg·s-1,P>0.05),房颤发生后则明显降低(129±15vs79±14 mm Hg,P<0.05;2434±531vs1636±417 mm Hg·s-1, -2707±714vs-1318±237 mm Hg·s-1,P<0.05)。结论 以ACh+心房快速起搏制备的犬急性房颤模型具有诱发率高、房颤持续时间较长、安全和制作简单的特点,房颤的机制及对心功能的影响可部分模拟临床上房颤的病理生理过程。
Abstract:
AIM To investigate the effect and mechanism of acetylcholine(ACh) in inducing canine acute atrial fibrillation(AF) model. METHODS It was performed on 10 mongrel dogs. In this study,intravenous(IV) infusion of ACh and atrial burst-pacing were performed on the dogs,then the dose of ACh was increased gradually according to the duration of AF until sustained AF(lasting≥3 min) could be induced.Sinus rate,corrected sinus-atrial recovery time(CSANRT) and atral effective refractive period(ERP) were measured before and after ACh,and pressure of left ventricle, maximal increasing and decreasing velocity of left ventricle' s pressure were also measured before and after ACh and after AF was induced. RESULTS Before the ACh,sustained AF was failed to be induced by atrial burst-pacing. With the increasing of the dose of ACh, the duration of AF prolonged. When the ACh increasing to a certain dose(18±8μg·kg-1·min-1), sustained AF could be induced in all ten dogs by atrial burst-pacing and AF occurred spontaneously in one dog. The mean duration was 344±173 s. Sinus rate droped markedly(157±25vs138±20·min-1,P<0.01), corrected sinus-atrial recovery time( CSANRT) was prolonged(44±13vs81±24 ms,P<0.01) and atrial ERP was shortened(80±16vs60±13 ms,P<0.01). Pressure of left ventricle, maximal increasing and decreasing velocity of left ventricle' s pressure had no significant differences before and after ACh(140±29vs129±15 mm Hg·s-1,P>0.05;2752±876vs2434±531 mm Hg·s-1; -3115±1067vs-2707±714 mm Hg·s-1,P>0.05),but decreased significantly after AF was induced(129±15vs79±14 mm Hg,P<0.05;2434±531vs 1636±417 mm Hg·s-1, -2707±714vs-1318±237 mm Hg·s-1,P<0.05).CONCLUSION The model is highly reproducible,and sustained AF was readily and safely inducible for a prolonged period,which can partly imitate the physiopathological procedure of clinical AF.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2001-08-22.
更新日期/Last Update: 2005-05-01