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渗透压对大鼠心室肌细胞L 型Ca2+ 电流的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第4期
页码:
281-283,286
栏目:
实验研究
出版日期:
2002-07-01

文章信息/Info

Title:
Effect of osmotic changes on L-type calcium channel currents in isolated ventricular myocytes
作者:
董　玲1;  周士胜1;  臧益民1;  高　瞻1;  杨安钢2;  王跃民1;  马　恒1
第四军医大学: 1.生理学教研室, 2.生物化学与分子生物学教研室, 陕西 西安 710032
Author(s):
DONG Ling1;  ZHOU Shi-sheng1;  ZANG Yi-min1;  GAO Zhan1;  YANG An-gang2;  WANG Yao2
1.Department of Physiology, 2.Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, Shaanxi 710032, China
关键词:
L 型钙电流;  心室肌细胞;  高渗透性皱缩;  低渗透性肿胀;  大鼠
Keywords:
L-type Ca2+ current;  ventricular myocyte;  Hyperotonic shrinkage;  Hypotonic swelling;  rat
分类号:
Q463
DOI:
-
文献标识码:
A
摘要:
目的 观察细胞胞外渗透压的改变对大鼠心室肌L 型Ca2+ ( ICa,L ) 电流的影响。方法 采用全细胞膜片钳技术记录大鼠心室肌细胞ICa,L。结果 细胞外的渗透压由310mOsm/kg H2O 降低至220mO sm/kg H2O 可使ICa,L 的rundown 明显变慢, 电流大小无统计学意义上的差异; 而当其中细胞外的渗透压由310 mOsm/kg H2O 升高至410mOsm/kg H2O 时可使ICa,L 减小(51.4±7.9)% (n= 4, P< 0.01)。结论 细胞外渗透压变化对大鼠心室肌基础ICa,L有明显影响。
Abstract:
AIM To investigate the effect of osmotic changes on the L-type calcium channel currents in isolated ventricular myocytes. METHODS L-type Ca2+current was recorded using the whole cell voltage clamp technique.RESULTS Lowering osmolarity to 220 mOsm/kg H2O did not significantly increased the amplitude of the ICa,L, but the run down of the current was slow. Hyperotonic solution (410 mOsm/kg H2O) reduced ICa,Lamplitude (51.4±7.9)% (n=4,P<0.01).CONCLUSION These results indicate that the changes in osmolarity of rat ventricular myocytes can modulate the function of L-type calcium channel.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2001-12-01.基金项目: 本课题由国家自然科学基金(No. 39870381)、国家教委留学回国人员启动基金, 长江学者匹配骨干教师基金和军队杰出中轻年人才基金资助
更新日期/Last Update: 2002-07-01