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缺氧与缺氧/复氧诱导心肌细胞凋亡的比较及意义(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第5期
页码:
369-371
栏目:
基础研究
出版日期:
2002-09-01

文章信息/Info

Title:
Comparison of hypoxia and hypoxia-reoxygenation induced cardiomyocytes apoptosis
作者:
张 峰1;  罗晓星1;  张 涛2;  刘丽宏1;  李 晨1;  谢建军1; 赵德化1
第四军医大学:1.药理学教研室,陕西西安710032,2.唐都医院胸外科,陕西西安710038
Author(s):
ZHANG Feng1; L UO Xiao-xing1;  ZHANG Tao2;  LIU Li-hong1;  LI Chen1;  XIE Jian-jun1;  ZHAO De-hua1
1.Department of Pharmacology,Fourth Military Medical University,Xi' an, Shaanxi 710032,China
关键词:
心肌细胞; 缺氧; 缺氧/复氧; 凋亡
Keywords:
cardiomyocyte; hypoxia; hypoxia-reoxygenation; apoptosis
分类号:
R349.5
DOI:
-
文献标识码:
A
摘要:
目的 研究单纯缺氧与缺氧复氧对体外培养的新生大鼠心肌细胞凋亡的影响,探讨凋亡在心肌细胞缺氧/复氧损伤中的作用。方法 取体外培养的新生大鼠心肌细胞,分两组,均置于950 ml/L N2,50 ml/L CO2孵箱中培养16,32,48 h,其中一组缺氧后再恢复正常条件培养6h,分别造成缺氧和缺氧/复氧损伤的细胞模型,TUNEL染色观察凋亡细胞形态学变化,流式细胞仪检测心肌细胞凋亡率。结果 心肌细胞经历缺氧和缺氧/复氧损伤后,TUNEL染色可见凋亡阳性细胞;应用流式细胞仪定量检测,心肌细胞缺氧培养16,32,48 h后,其凋亡率分别为: (2.9±0.5)%,(6.2±0.8)%和(26.6±3.0)%;心肌细胞在缺氧16,32和48 h后复氧6 h,其凋亡率分别为:(5.5±0.7)%,(11.0±1.1)%和(14.2±1.6)%。结论 心肌细胞凋亡率随着缺氧时间的延长而增高;缺氧/复氧较单纯缺氧可进一步加重心肌细胞的损伤。
Abstract:
AIM To compare hypoxia and hypoxia-reoxygenation induced apoptosis of cardiomyocytes and to investigate the role of apoptosis in cardiomyocytes injury caused by hypoxia/reoxy-genation.METHODS Cultured neonatal rat cardiomyocytes were divided into two groups.Both groups were cultured in an incubator of 950 ml/L N2and 50 ml/L CO2for 16, 32 and 48 h. Cells of one group were put into normal incubation for 6 h after hypoxia to form the cell models of hypoxia-reoxygenation injury. Morphological changes in apoptotic cardiomyocytes were measured by TUNEL staining. Apoptosis rates were measured by flow cytometer.RESULTS Positive cells were detected by TUNEL staining. Apoptotis rates of cardiomyocytes measured by flow cytometer after hypoxia for 16,32 and 48 h were (2.9±0.5)%,(6.2±0.8)% and (26.6±3.0)% respectively.The apoptosis rates of cells undergoing hypoxia for 16,32 and 48 h followed by reoxygenation for 6 h were (5.5±0.7)%,(11.0±1.1)% and (14.2±1.6)% respectively.CONCLUSION The apoptosis rates of cardiomyocytes increased with time of hypoxia.Reoxygenation could worsen cardiomyocytes injury,as compared with hypoxia.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-03-25.基金项目：军队科学基金面上课题 (No.96M100)
更新日期/Last Update: 2002-09-01