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|本期目录/Table of Contents|

失血性休克时大鼠心脏G蛋白含量的变化及其意义(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第6期
页码:
474-476
栏目:
实验研究
出版日期:
2002-11-01

文章信息/Info

Title:
Expression and the effects of cardiac G protein during hemorrhagic shock in rats
作者:
刘 苹; 熊仁平; 周元国
第三军医大学大坪医院野战外科研究所分子生物学中心,重庆400042
Author(s):
LIU Ping;  XIONG Ren-ping;  ZHOU Yuan-guo
Molecular Biological Center,Research Institute of Surgery,Daping Hospital,Third Military Medical University,Chongqing 400042, China
关键词:
腺苷酸环化酶; 休克; 出血性; 心肌;  G蛋白; 信号转导
Keywords:
adenyl cyclase; shock; hemorrhagic;  myocardium;  G protein;  signal transduction
分类号:
R541.64
DOI:
-
文献标识码:
A
摘要:
目的 研究失血性休克时大鼠心脏G蛋白含量及功能的变化。方法 大鼠失血性休克,记录平均动脉压,呼吸频率及失血量,各时相点血气分析;免疫印迹法测定大鼠心脏Giα和Gsα含量。结果 休克6 h,心脏Gsα含量(第一条带19772±1186像素,第二条带20865±804像素)较正常对照组(第一条带23583±714像素,第二条带24777±690像素)下降(P<0.05,P<0.01);Giα含量(27767±801像素)较正常对照组(17353±799像素)升高(P<0.01)。结论 心脏Gsα含量下降和Giα含量升高可能是导致细胞膜AC信号转导功能障碍和心功能抑制的重要原因。
Abstract:
AIM To study the alteration of the content of G-proteinsαsubunits in rats in hemorrhagic shock. METHODS Levels of Giαand Gsαin sarcolemma were detected by Western Blot analysis,and recorded MAP,RR and blood-loss volume as well.RESULTS In the ventricles, the contents of two Gsαband in the group HS was significantly lower than that in the group NC(P<0.05,P<0.01).The content of one Giαband in the group HS was significantly higher than that in the group NC(P<0.01).CONCLUSION Alteration of cardiac G protein may contribute to the dysfunction of adenyl cyclase signal transduction system and heart during hemorrhagic shock.

参考文献/References

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[6]王 瑶,吴立铃,葛明珠.缺血-再灌注时大鼠心脏Gi蛋白α亚基的变化[J].生理学报,1998,50(5):514-518.

[7] Ohyanagi M,Yamamoto J,Nakamura K,et al. Messenger RNA for the guanine nucleotide-binding regulatory protein ( Gprotein)is reduced in the acute ischemic myocardium[J].J Mol Cell Cardiol,1995,27:1131-1139.

[8] Niroomand F,Weinbrenner C,Weis A,et al. Impaired function of inhitory G proteins during acute myocardial ischemia of canine hearts and its reversal during reperfusion and a second period of ischemia[J].Circ Res,1995,76:861-870.

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备注/Memo

备注/Memo:
收稿日期：2001-11-05.
更新日期/Last Update: 2002-11-01