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ox-LDL对人血单核细胞组织基质蛋白抑制物表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第2期
页码:
97-99,103
栏目:
实验研究
出版日期:
2003-03-01

文章信息/Info

Title:
Effect of ox-LDL on expressions of TIMPs in human monocytes
作者:
王长谦; 汤大呜; 黄定九; 王彬尧; 王利民; 宋伟; 谢秀兰; 徐依敏; 丁泓毅
上海第二医科大学附属仁济医院心内科,上海 200001
Author(s):
WANG Chang-qian; TANG Da-ming; HUANG Ding-jiu; WANG Bin-yao; WANG Li-min;  SUNG Wei; XIE Xiu-lan; XU Yi-min; DING Hong-yi
Department of Cardiology,Renji Hospital Affiliated to shanghai Second Medical Vniversity, Shanghai,200001,China
关键词:
血管生长因子; 组织基质金属蛋白酶抑制物; 动脉粥样硬化
Keywords:
angiogensesis factor; tissue in hibitors of metalloproteinase; atherosclerosi
分类号:
Q513
DOI:
-
文献标识码:
A
摘要:
目的:探讨氧化修饰低密度脂蛋白(ox-LDL)对人单核细胞源巨噬细胞(HMDM)组织基质金属蛋白酶抑制物 (TIMPs)的基因和蛋白表达的影响。方法:分别应用RT-PCR和Westernblot检测TIMPs(TIMP-1和TIMP-2)基 因和蛋白表达。结果:ox-LDL可明显抑制HMDM TIMP-1mRNA及其蛋白的表达,但对TIMP-2mRNA及其蛋 白的表达无明显影响。结论:ox-LDL可抑制HMDM TIMP-1mRNA及其蛋白的表达,可能间接增强基质金属蛋 白酶(MMPs)活性。
Abstract:
AIM:To observe the effects of ox-LDL on the expressions of TIMPs (Tissue In hibitors of Metalloproteinase).METHODS:The gene and protein expressions of TIMPs(TIMP-1and TIMP-2)were respectively detected byRT-PCR and Western blot. RESULTS:ox-LDL can obviously decrease the expressions of TIMP-1 gene and its protein in human monocytes,but does not affect the expression of TIMP-2.CONCLUSION:Ox-LDL can decrease the expressions of TIMP-1 gene and its protein in human monocytes

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-1-18.
更新日期/Last Update: 2003-03-01