我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

低浓度强心甙对豚鼠心室肌细胞Na+/K+泵电流的激活作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第4期
页码:
300-302
栏目:
实验研究
出版日期:
2003-07-01

文章信息/Info

Title:
Stimulative effect of low concentration dihydroouabain on Na+/K+ pump current in guinea pig ventricular myocytes
作者:
尹京湘; 王永利; 李清; 丁延峰; 苏素文
河北医科大学基础医学研究所药理室,河北 石家庄 050017
Author(s):
YIN Jing-xiang;  WANG Yong-li;  LI Qing;  DING Yan-feng;  SU Su-wen
Department of Pharmacology, Institute of Basic Medicine, Hebei Medical University,Shijiazhuang, Hebei 050017, China
关键词:
双氢哇巴因(DHO); 钠/钾泵电流; 豚鼠心肌细胞; 膜片钳术; 全细胞
Keywords:
dihydroouabain(DHO);  Na+/K+ pump current(Ip); guinea pig myocytes;  patch clamp techniques;  whole-cell
分类号:
R963
DOI:
-
文献标识码:
A
摘要:
目的:解释治疗水平(低浓度)强心甙的正性肌力作用。方法:用全细胞膜片钳技术,在分离的豚鼠心室肌细胞观察到双氢哇巴因(DHO)对Na+/K+泵电流(Ip)的激活作用。结果:DHO在10-8～10-10mol·L-1范围内可增加外向电流。当从细胞外液去除K+或从电极内液去除Na+或胞内应用vanadate抑制了Ip后,外向电流消失。结论:DHO可使Na+/K+泵兴奋,产生了外向泵电流。Ip激活是低浓度强心甙对心脏Na+/K+泵的直接作用。
Abstract:
AIM: To explain the positive inotropic action of glycosides at therapeutic levels. METHODS: Using the whole-cell patch clamp technique, we studied the effects of low concentration dihydrooubain (DHO) on Na+/K+ pump current (Ip) in isolated guinea pig ventricular myocytes. RESULTS: An increase in the outward current was observed with DHO concentrations ranging from 10-10 to 10-8mol·L-1. The increase did not occur when Ip was blocked by the removal of either external K+ or intracellular Na+, or by the addition of intracellular vanadate. CONCLUSION: DHO could stimulate Na+/K+ pump, and generate the outward pump current. The stimulation of Ip is a directaction of low concentration glycosides on the cardiac Na+/K+ pump.

参考文献/References

[1] Gao J, Mathias IS, Cohen IS, et al. Two functionally distinct Na/K pumps in cardiac ventricular myocytes [J] . J Gen Physiol, 1995, 106:995.

[2] Noble D. Mechanism of action of therapeutic levels of cardiac glycosides [J] . Cardiovasc Res, 1980, 14:495.

[3] Geering K. Sub unit assembly and functional maturation of Na/K ATPase [J] . J Membr Biol, 1990, 115:109.

[4] Wang Y, Gao J, Mathias RT, et al. α-adrenergic effects on Na+-K+ pump current in guinea pig ventricular myocytes [J] . J Physiol, 1998, 509:116.

[5] Gao J, Wymore R, Wymore RT, et al. Isoform-specific regulation of the sodium pump by α-andβ-adrenergic agonists in guinea pig ventricles [J] . J Physiol, 1999, 516(2):377.

[6] Wang Y, Wymore R, Gao J, et al. α and β adrenergic are coupled to different Na+/K+ pump isoformsin guinea pig ventricular myocytes [J] . Biophys J, 1998, 74:A161.

[7] Hougen TJ, Spicer N, Smith TW. Stimulation of monovalent cation active transport by low concentrations of cardiac glycosides. Role of catecholamines [J] . J Clin Invest, 1981, 68(5):1207.

[8] Xie Z. Ouabain interaction with cardiac Na/K-ATPase reveals that the enzyme can act as a pump and asasignal transducer [J] . Cell Mol Biol(Noisy-le-grand), 2001, 47(2):383.

备注/Memo

备注/Memo:
收稿日期：2002-06-12.
更新日期/Last Update: 2003-07-01