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|本期目录/Table of Contents|

Fas配体基因导入对大鼠颈动脉损伤后新生内膜的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第4期
页码:
308-310
栏目:
实验研究
出版日期:
2003-07-01

文章信息/Info

Title:
Effects of adenoviral vector-mediated Fas ligand gene introduction on neointima after rat carotid injury
作者:
陶红1; 陈立东1; 黄从新2; 张群林1; 王家宁1; 黄永章1
1.郧阳医学院附属太和医院心内科,湖北 十堰 442000;2.武汉大学人民医院心内科,湖北 武汉 430060
Author(s):
TAO Hong1;  CHEN Li-dong1;  HUANG Cong-xin2;  ZHANG Qun-lin1;  WANG Jia-ning1;  HUANG Yong-zhang1
1.Department of Cardiology, Affliated Taihe Hospital, Yunyang Medical College, Shiyan, Hubei 442000, China
关键词:
Fas配体; 血管内膜增生; 腺病毒; 基因疗法
Keywords:
Fas ligand;  neointimal hyperplasia;  adeno virus vecter;  gene therapy
分类号:
Q789
DOI:
-
文献标识码:
A
摘要:
目的:探讨腺病毒载体介导Fas配体(FasL)基因导入对大鼠颈动脉损伤后新生内膜的影响。方法:利用重组腺病毒载体将FasL导入大鼠颈动脉球囊损伤的内膜,14d后观察其对新生内膜形成的影响。结果:通过腺病毒载体介导导入FasL基因的被球囊损伤的大鼠颈动脉,与Ad-βgal基因的对照组相比较,14d后损伤血管新生内膜/中膜面积比降低了73%(P<0.01)。结论:FasL可抑制新生内膜的增生。
Abstract:
AIM: To study the effects of Fas ligand (FasL) gene transfer mediated by adeno viral vector on neointimal hyperplasia after rat carotid injury. METHODS: Restenosis model was established in SD rat and FasL gene was transduced into the carotid artery by adeno viral vector after carotid balloon injury and neointimal/media area ratio in local artery 14 days after gene tranafer was measured. RESULTS: FasL gene was successfully transducted into the carotid artery after balloon injury. Neointimal/media area ratio was reduced 73% (P<0.01) compared with the control group β gal 14 days after gene transfer. CONCLUSION: FasL gene transfer to the vessel wall inhibits neointimal formation. It is an effective gene therapy to restenosis.

参考文献/References

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[8] Sara M, Perlman H, Muruve DA, et al. Fas Ligand gene transfer to the vessel wall inhibits neointimal formation and overrides the adenovirus-mediated T cell response [J]. Pro cNad Acad USA, 1998, 95:1213-1217.

[9] 孙宝华. 细胞凋亡信号转导中的蛋白水解酶 [J] , 国外医学*分子生物学分册, 1999, 21(1):1-5.

备注/Memo

备注/Memo:
收稿日期：2002-04-15.
更新日期/Last Update: 2003-07-01