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小鼠心室肌细胞容积敏感性Cl-电流的特性及调节(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第5期
页码:
388-393
栏目:
实验研究
出版日期:
2003-09-01

文章信息/Info

Title:
Characteristics and modulation of volume-activated Cl-current in ventricular cardiomyocytes isolated from adultm ice
作者:
龚卫琴12; 臧益民2; 徐洪涛3; 王晓明12; 李源1
第四军医大学:1.西京医院老年病1科,2.生理学教研室,陕西 西安 710032;3.海军总医院心血管内科
Author(s):
GONG Wei-qin12;  ZANG Yi-min2;  XU Hong-tao3;  WANG Xiao-ming12;  LI Yuan1
1.Department of Geriatrics, Xijing Hospital,2.Department of Physiology, Fourth M ilitary Medical University, Xian, Shaanxi 710032; 3. Department of Cardiovascular Disease, Beijing Navy Hospital
关键词:
心室肌细胞; 全细胞记录; 容积敏感性Cl-通道; PKC
Keywords:
cardiomyocytes;  patch clamp;  volume-sensitive Cl-channel;  PKC
分类号:
R331.3
DOI:
-
文献标识码:
A
摘要:
目的:研究小鼠心室肌细胞容积敏感性Cl- 电流的电生理学特性以及蛋白激酶C (PKC) 调节机制。方法:膜片钳全细胞记录法记录分离的C57BL ?6 小鼠心室肌细胞低渗诱导的容积敏感性Cl- 电流的变化。结果:小鼠心室肌细胞存在典型的容积敏感性Cl- 电流, 其呈现外向整流性、高电位刺激下的时间依赖性失活、I- > B r- > Cl- 的可通透性和Cl- 通道阻断剂(D IDS) 敏感的电生理学特性。而且, PKC 激动剂(PMA ) 明显有助于该电流的激活。结论:小鼠心室肌细胞的容积敏感性和外向整流性的Cl- 电流受到PKC 激动剂的正向调节。
Abstract:
AIM: To investigate the electrophysiological characteristics of volume-activated Cl- curren t ( ICl, vol) inventricular cardiomyocytes isolated from adult mice and the modulation mechanism of protein kinase C (PKC). METHODS: ICl, vol was recorded by using whole cell voltage clamp technique and PKC was activated by phorbol 12-myristate 13-acetate (PMA). RESULTS: Whole-cell recordings indicated that mouse cardiomyocytes had typical volume-sensitive Cl- current which exhibited moderate outward rectification, time-dependent in activation, I- > B r- > Cl-permeability sequence, and the sensitivity of 4, 4’-diisothiocyanato stilbene-2, 2’-disulfonic acid (DIDS). We also observed that PMA obviously and quickly enhanced volume-sensitive Cl- current. CONCLUSION: Volume-sensitive outwardly rectifying (VSOR ) Cl- current exists in mouse cardiomyocytes and was up-regulated by PKC activator.

参考文献/References

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[2] Jentsch TJ , Stein V , Weinreich F, et al. Molecular structure and physiological function of chloride channels [J] . Physiol Rev, 2002, 82:503- 568.

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备注/Memo

备注/Memo:
收稿日期：2002-11-22.
更新日期/Last Update: 2003-09-01