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槲皮素、异鼠李素对体外低密度脂蛋白氧化修饰的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第5期
页码:
414-416
栏目:
实验研究
出版日期:
2003-09-01

文章信息/Info

Title:
Effects of quercetin and isorhamnetin on oxidative modification of LDL induced by Fe2+ in vitro
作者:
李家富1; 何涛2; 邓辉胜1; 罗兴林1; 魏宗德1
泸州医学院:1.附属医院心血管病研究室, 2.生物化学教研室,四川 泸州 646000
Author(s):
LI Jia-fu1;  HE Tao2;  DENG Hui-sheng1;  LUO xing-lin1;  WEI Zong-de1
Department of Cardiology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China
关键词:
低密度脂蛋白; 氧化修饰; 槲皮素; 异鼠李素; 动脉粥样硬化
Keywords:
low density lipoprotein;  oxidative modification;  quercetin;  isorhamnetin;  atherosclerosis
分类号:
Q513.5;Q591.2　
DOI:
-
文献标识码:
A
摘要:
目的:观察槲皮素(Que)、异鼠李素(Iso) 在体外对Fe2+介导的人血浆低密度脂蛋白(LDL)氧化修饰的影响。方法: 采用一次性密度梯度法分离正常人血浆LDL,在体外用Fe2+进行氧化修饰。抗氧化组在温育前加入不同浓度Que或Iso(50～ 200 Lmo l·L - 1)。通过检测脂蛋白中丙二醛(MDA)、维生素E(VitE) 及超氧化物歧化酶(SOD)活性, 比较LDL受氧化程度。结果: Que和Iso明显减少OX-LDL中MDA 含量(P<0. 01) ,并呈现一定的浓度效应, 同时明显延缓内源性抗氧化物质VitE含量降低, 显著提高SOD活性(P<0. 01)。结论:Que和Iso均对Fe2+介导的体外LDL氧化修饰具有明显的抑制作用, 其作用途经可能与抗自由基氧化活性有关。
Abstract:
AIM: To observe the effects of quercet in (Que) and isorhamnetin (Iso) on the oxidative modification of low density lipoprotein (LDL). METHODS: Human LDL was prepared by on-step ultracentrifuge and oxidized by Fe2+ in vitro. Que and Iso in different concentration were added to LDL solution before incubation with Fe2+. The content of malondialdehyde(MDA), vitamin E (V it E) and activity of superoxide dismutase (SOD) in oxidized low density lipoprotein (ox-LDL ) were determined. RESULTS: Compared with ox-LDL group, Que and Iso (50～200L mo l·L-1)significantly inhibited MDA production in a dependant concentration maner. Que and Iso alsoelevated vitE level and SOD activities markedly (P<0.01). CONCLUSION: Que and Iso obviously inhibite oxidative modification of LDL induced by Fe2+. The mechanism of the inhibitory effect of both Que and Iso on oxidative modification of LDL may be associated with the inhibition of generation of oxygen free radicals and with the prevention of inactivation of SOD.

参考文献/References

[1] Witztum JL, Steinberg D. Role of oxidized low density lipoprotein in atherogensis [J]. J Clin Invest, 1991, 88:1785-1792.

[2] 陈奇, 张士善. 槲皮素的药理研究及临床应用 [J] . 中华医学杂志, 1977, 57(8):512-516.

[3] 康德伸. 醋柳黄酮治疗缺血性心脏病的作用 [J] . 老年医学杂志, 1994, 14(1):61-63.

[4] 张华林, 刘秉文. 一次性密度梯度超速离心分离人血清脂蛋白 [J] . 生物化学与生物物理学报, 1989, 21(3):257-260.

[5] Henriksen T, Mahoney EM, Steinberg D. Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: recognition by receptors for acetylated low density lipoproteins [J] . Proc Natl Acad Sci USA , 1981, 78:6499-6503.

[6] Heinecke JW, Rosen H, Chait A. Iron and copper promote modification of low density lipoprotein by human arterial smooth muscle cells in culture [J] . J Clin Invest, 1984, 74:1890-1894.

备注/Memo

备注/Memo:
收稿日期：2002-09-03.
更新日期/Last Update: 2003-09-01