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低氧对家兔心脏成纤维细胞增殖与表型的影响 (PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第3期
页码:
209-211
栏目:
实验研究
出版日期:
2004-05-01

文章信息/Info

Title:
Effect of hypoxia on the proliferation and phenotype of cardiac fibroblasts from rabbits
作者:
王映梅; 李青; 闰庆国; 惠延平; 马福成; 胡沛漆
第四军医大学病理学教研室，陕西 西安 710032
Author(s):
WANG Ying-mei;  LI Qing; YAN Qing-guo; HUI Yan-ping;  MA Fu-cheng; HU Pei-zhen
Department of Pathology，Fourth Military Medical University，Xi'an，Shaanxi 710032，China
关键词:
低氧; 成纤维细胞; 心脏; 表型; 从动蛋白
Keywords:
hypoxia; cardiac fibroblasts; phenotype; α-actin
分类号:
R331. 31
DOI:
-
文献标识码:
A
摘要:
目的:观察不同程度低氧对家兔心脏成纤维细胞(CFs)增殖与表型的影响。方法:分离、纯化、培养家兔Us,随机分为3组:对照组、中度低氧组和重度低氧组，以四哇盐(MTT)比色法检测细胞的增殖情况，采用激光共聚焦显微镜检测各组细胞内肌动蛋白α-actin的表达。结果:中度低氧组MTT的490nm吸光值(A490 )为0.46±0.05较对照组(0.37±0.04)显著增加(P<0.05 ,n=8)，而重度低氧组A490，值为0.31±0.03较对照组显著降低(P<0.05 ,n=8)。激光共聚焦显微镜结果显示，对照组CFs内无明显的。α-actin表达，荧光值为1127±120。中度低氧组细胞内。α-actin表达显著增加，荧光值为1672±193，与对照组比差异显著(P<0.05，n=4)。重度低氧组细胞内α-actin表达较少，荧光值为1355±231，与对照组比无显著差异(P>0.05,n=4),结论:中度低氧促进家兔Us增殖，并且出现肌成纤维细胞的表型特点，而重度低氧具有细胞毒性。
Abstract:
AIM:To investigate the effect of hypoxia on the proliferation and phenotype of cardiac fibroblasts(CFs) from rabbits. METHODS:The purified cardiac fibroblasts were cultured and divided randomly into three groups:control group，moderate hypoxia (MH) group and severe bypoxia (SH) group. 72 h later，MTT method was used to investigate the proliferation of CFs，and α-actin in cardiac fibroblasts was measured by means of laser scanning confocal microscopy. RESULTS: MTT A490，value of the MH group (0.46±0.05) was significantly higher than that of control group (0. 37±0.04) (P

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备注/Memo

备注/Memo:
收稿日期：2003-8-1
更新日期/Last Update: 2004-05-01