我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

尾加压素II 介导心肌细胞肥大与其和钙离子的关系(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
414-416,420
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
Urotensin Ⅱinduced hypertrophy in cultured cardiac myocytes and the relation with calcium
作者:
陈军红; 赵连友; 郑强荪; 薛玉生; 陈永清; 王　斌
第四军医大学唐都医院心内科,陕西西安710038
Author(s):
CHEN Jun-hong ;  ZHAO Lian-you ;  ZHENG Qiang-sun ;  XUE Yu-sheng ;  CHEN Yong-qing ;  WANG Bin
Department of Cardiology ,Tangdu Hospital ,Fourth Military Medical University ,Xi’an ,Shaanxi 710038 ,China
关键词:
尾加压素Ⅱ; 心肌细胞; 肥大; 钙离子
Keywords:
urotensin Ⅱ; cardiac myocyte ; hypertrophy ; calcium
分类号:
Q71 ;R542. 2
DOI:
-
文献标识码:
A
摘要:
目的: 观察尾加压素Ⅱ(U Ⅱ) 对心肌细胞肥大的影响及其与钙离子的关系,为防治高血压左室肥厚提供理论 依据。方法:以培养的SD 乳鼠心肌细胞为实验模型,通过测定心肌细胞表面积、蛋白含量和蛋白合成速率作为心 肌细胞肥大的指标,应用激光共聚焦显微镜检测U Ⅱ作用后细胞内游离钙的变化。结果: ①随着U Ⅱ浓度的增加, 心肌细胞表面积呈剂量依赖性增加,其中10 - 8和10 - 7 mol/ L U Ⅱ组心肌细胞表面积分别为2046 ±268 和3662 ±259 μm2 ,均明显高于对照组的(936 ±100μm2) ,差异非常显著( P < 0. 01) 。②随着U Ⅱ浓度的增加,心肌细胞的蛋白含 量和蛋白合成速率呈递增趋势,其中10 - 8和10 - 7 mol/ L U Ⅱ组心肌细胞蛋白含量分别为1. 6 ±0. 4 和1. 9 ±0. 3 ng/ cell ,明显高于对照组(0. 8 ±0. 2 ng/ cell) ,差异非常显著( P < 0. 01) 。蛋白合成速率分别为2508 ±299 和2898 ±625 cpm/ well ,与对照组的(1026 ±92 cpm/ well) 比较,差异非常显著( P < 0. 01) 。③加入10 - 7 mol/ L U Ⅱ后心肌细胞内钙 离子荧光强度迅速升高(99. 0 ±14. 1) ,与对照组(34. 6 ±5. 8) 相比差异非常显著( P < 0. 01) 。钙离子浓度由对照组 的(288 ±159) 上升至(958 ±664) ,二者之间差异亦非常显著( P < 0. 01) 。结论:U Ⅱ可以诱导MC 的肥大,这可能与 钙离子浓度的升高有关。
Abstract:
AIM:To observe the hypertrophy effect induced by urotensin Ⅱ(U Ⅱ) on cultured cardiomyocytes and the relation with calcium. METHODS:The effect of U Ⅱon cardiac myocyte (MC) hypertrophy was assessed by measurement of cell surface area , protein content and 3H-leucine incorporation , the U Ⅱ-induced changes of Ca2 + in the MC were observed by means of laser scanning confocal microscope (LSCM) . RESULTS: ①U Ⅱincreased MC surface area in a dose dependent manner. After 10- 8and 10 - 7 mol/ L U Ⅱwere added ,the cell surface areas were 2046 ±268 and 3662 ±259μm2 , respectively ,significantly higher than the surface area of the control ( P < 0. 01) . ②10 - 8 and 10 - 7 mol/ L U Ⅱincreased 3H2leucine incorporation also in a dose dependent manner. The protein content were 1. 7 ±0. 4 and 1. 9 ±0. 3 (ng/ cell) respectively , and 3Hleucine incorporations were 2508 ±299 and 2898 ±625 (cpm/ well) respectively. They were all significantly higher than those of the control ( P < 0. 01) . ③A rapid increase of Ca2 + in MC was observed shortly after 10 - 7 mol/ L U Ⅱwas added in cultured MC. CONCLUSION: U Ⅱcan induce cardiac myocyte hypertrophy and increase of calcium ,and calcium maybe was the key factor in the hypertrophy effect induced by UⅡ,which could be useful for the prevention of left ventricular hypertrophy.

参考文献/References

[1 ] Martin A ,Hefti , Beatrice A , et al . Hans M. Signaling pathways in cardiac myocyte hypertrophy [ J ] . J Mol Cell Cardiol , 1997 , 29 ; 2873 -2892.

[2 ] Coulouarn Y, Lihrmann I , Jegou S , et al . Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord[J ] . Proc Natl Acad Sci USA ,1998 ,95(26) :15803 - 15808.

[3 ] Liu Q , Pong SS , Zeng Z , et al . Identification of urotensin II as the endogenous ligand for the orphan G2protein2coupled receptor GPR14 [J ] .Bichem Biophys Res Commun ,1999 ,266 :174 - 178.

[4 ] Russell FD , Meyers D , Galbraith AJ , et al . Elevated plasma levels of human urotensin II immunoreactivity in congestive heart failure[J ] . Am J Physiol Heart Circ Physiol ,2003 ,285 (4) :H1576 - H1581.

[ 5 ] Ames RS ,Sarau HM,Chambers JK, et al . Human urotensin II is a potent vasoconstrictor and agonist for the orphan receptor GPR14 [J ] . Nature ,1999 ,401 :282 - 286.

[6 ] Katano Y, Ishihata A , Aita T. Vasodilator effect of urotensin Ⅱ,one of the most potent vasocontricting factors ,on rat coronary arteries[J ] . Eur J Pharmacol ,2000 ,402 :209 - 211.

[7 ] 张勇刚,陈亚红,马春艳,等. 尾加压素的促丝裂作用[J ] . 中国动脉硬化杂志,2001 ,9(1) :14 - 16.

[8 ] Zhu W,Zou Y,Shiojima I , et al . Ca2 + / calmodulin-depent kinase Ⅱand calcineurin play critical roles in endothelin-1-induced cardiomycyte hypertrophy[J ] . J Biol Chem ,2000 ,275(20) :15239 - 15245.

[9 ] Gibson A. Complex effects of Gillichthys urotension Ⅱon rat aortic strips [J ] . Br J Pharmacol ,1987 ,91(1) :205 - 212.

[10 ]郝胜利,张孙曦,唐朝枢,等. 牛璜酸对H2O2 引起的大鼠血管平滑肌细胞核[Ca2 + ]及胞浆[Ca2 + ]变化的影响[J ] . 中国药理学通 报,2001 ,17(1) :61 - 66.

[11 ]张正浩,张孙曦,李菊香. 钙信号在尾加压素- Ⅱ促血管平滑肌细胞中的增殖[J ] . 北京大学医学报,2002 ,34(3) :261 - 265.

备注/Memo

备注/Memo:
收稿日期：2003-10-13
更新日期/Last Update: 2004-09-01