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阿米洛利对心肌复极的影响及离子机制研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
410-413
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
Effects and ionic mechanisms of Amiloride on repolarization in myocytes
作者:
梁黔生; 郑　智
华中科技大学同济医学院附属同济医院急诊科,湖北武汉430030
Author(s):
LIANG Qian-sheng ;  ZHENG Zhi
Department of Emergency Medicine , Tongji Hospital , Tongji Medical College of Huazhong University of Science and Technology , Wuhan ,Hubei 430030 , China
关键词:
阿米洛利; 动作电位; 电压依赖性钾通道电流; 抗心律失常
Keywords:
Amiloride ;  action potential ;  potassium current ;  antiarrhythmia
分类号:
R331. 38 　　　
DOI:
-
文献标识码:
A
摘要:
目的: 研究阿米洛利(Amiloride) 对心肌复极的影响及离子机制。方法: 用标准微电极技术记录阿米洛利对 豚鼠乳头状肌细胞动作电位的影响; 用膜片钳技术记录电压依赖性的钾电流。结果: 10 μmol/ L、100 μmol/ L Amiloride 均可降低豚鼠乳头状肌动作电位幅度,延长动作电位时程;当累积浓度达100μmol/ L 时,Amiloride 轻微抑 制快激活延迟整流钾电流( Ikr) ,对慢激活延迟整流钾电流( Iks) 无影响。(1～100) μmol/ L 的Amiloride 浓度依赖性地 抑制内向整流钾电流( Ik1) 。结论:阿米洛利通过抑制电压依赖性的钾电流,延长复极以发挥抗心律失常的作用。
Abstract:
AIM: To study the effects and ionic mechanisms of Amiloride on repolarization in myocytes. METHODS:Action potentials of papillary muscle in isolated guinea pig was measured through standard and ion-selective microelectrodes. Voltage-dependent potassium currents in isolated guinea pig ventricular myocytes were recorded by using the whole-cell patch clamp techniques. RESULTS : In papillary muscle preparation of isolated guinea pig , 10μmol/ L 、100μmol/ L Amiloride prolonged 90 % action potential duration (248. 8 ±17. 5 ms of control to 270. 0 ±14. 7 ms and 317. 2 ±29. 3 ms) . 100μmol/ L Amiloride decreased action potential amplitude (120. 6 ±7. 1 mV vs 105. 4 ±2. 3 mV) ,maximal velocity of phase 0 depolarization(218. 6 ±8. 9 V/ S vs 162. 4 ±7. 0 V/ S) and 20 % action potential duration (101. 4 ±11. 6 ms vs 131. 6 ±17. 3 ms) . And resting potential depolarized slightly moved from ( - 79. 0 ±1. 8)mV to ( - 67. 4 ±5. 3)mV. Administrated up to 100μmol/ L in an accumulative manner , Amiloride slightly depressed Ikr . It had no effect on Iks . Amiloride (1～100μmol/ L) depressed Ik1 in a concentration2dependent manner. CONCLUSION:Amiloride prolonged duration of repolarization , depressed potassium currents ,which could give support to its uses in antiarrhythmia

参考文献/References

[1 ] Yamashita S , Motomura S , Taira N. Cardiac effects of amiloride in the dog[J ] . J Cardiovasc Pharmacol , 1981 , 3(4) : 704 - 715.

[2 ] Duff HJ , Brown CE , Cragoe EJ , et al . Antiarrhythmic activity of Amiloride : mechanisms [J ] . J Cardiovasc Pharmacol , 1991 ,17 (6) :879 - 88.

[3 ] Waldorff S , Hansen PB , Kjaergard H , et al . Amiloride-induced changes in digoxin dynamics and kinetics : abolition of digoxin-induced inotropism with amiloride[J ] . Clin Pharmacol Ther , 1981 ,30 (2) :172- 176.

[4 ] Dangman KH , Dresdner KP Jr , Michler RE. Transmembrane action potentials and intracellular potassium activity of baboon cardiac tissues[J ] .Cardiovasc Res , 1988 ,22 (3) :204 - 212.

[5 ] 梁黔生,程　岚,郑　智. 甲状腺素诱发大鼠心肌肥厚时瞬时外向钾电流、内向整流钾电流和钙电流的变化[J ] . 中华心律失常 学杂志,2001 ,5(1) :44 - 47.

[6 ] Sharma A , Singh M. Na ( + ) / H( + ) exchanger : An emerging therapeutic target in cardiovascular disorders [ J ] . Drugs Today ( Barc) ,2000 , 36 (11) :793 - 802.

[7 ] Masereel B , Pochet L , Laeckmann D. An overview of inhibitors of Na( + ) / H ( + ) exchanger [J ] . Eur J Med Chem ,2003 , 38 (6) :547 -554.

备注/Memo

备注/Memo:
收稿日期：2003-11-24
更新日期/Last Update: 2004-09-01