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胆固醇对大鼠心脏成纤维细胞增殖的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
423-426
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
Effects of cholesterol on the proliferation of cultured rat cardiac f ibroblasts
作者:
陈宝莹1; 魏经国1; 王耀程1; 杨向新1; 于　军2; 朱妙章2
第四军医大学:11 唐都医院放射科, 21 基础部生理学教研室,陕西西安710038
Author(s):
CHEN Bao-ying1; WEI Jing-guo1; WANG Yao-cheng1; YANG Xiang-xin1; YU Jun2; ZHU Miao-zhang2
1.Department of Radiology , Tangdu Hospital , 2. Department of Physiology ,Faculty of Preclinical Medical , Fourth Military Medical University , Xi’an , Shaanxi 710038 ,China
关键词:
胆固醇;  心肌纤维细胞;  c-fos ;  流式细胞仪
Keywords:
cholesterol ; cardiac fibroblasts ;  c-fos ;  flow cytometer
分类号:
R329. 25
DOI:
-
文献标识码:
A
摘要:
目的:研究胆固醇(CL) 对大鼠心脏成纤维细胞增殖的影响,并对其机制进行探讨。方法:分离、纯化、培养乳 鼠心脏成纤维细胞,完全随机分为3 组:对照组、中浓度胆固醇组(0. 5 g/ L) 和高浓度胆固醇组(1. 2 g/ L) 。以3H-TdR 掺入法测定各组细胞的增殖情况,并以流式细胞仪(FCM) 测定细胞周期。采用免疫组织化学染色方法观察各组cfos 的表达情况。结果:中浓度CL 可使培养的乳鼠心脏成纤维细胞3H-TdR 掺入量显著升高( P < 0. 05 vs 对照组) , 流式细胞仪检测表现为S 期细胞明显增多。高浓度CL 则抑制细胞生长,3H-TdR 掺入量显著降低( P < 0. 05 vs 对照 组) , S 期细胞数明显减少。同时中浓度CL 显著升高Fos 样免疫阳性细胞数及染色强度( P < 0. 05 vs 对照组) 。结 论:中等浓度胆固醇可促进大鼠心脏成纤维细胞增殖,其机制可能与影响细胞内c-fos 基因的表达有关。
Abstract:
AIM: To investigate the effect of cholesterol (CL) on the proliferation of the cardiac fibroblasts cultured from neonatal rats. METHODS: The cultured rat cardiac fibroblasts were divided randomly into three groups :control group , middle concentration group (0. 5 g/ L CL) and high concentration group (1. 2 g/ L CL) . The growth of rat cardiac fibroblasts in each group was measured by means of the incorporation of 3H-TdR and cell phases were determined by flow cytometer ( FCM) after the fibroblasts were affected by water-soluble cholesterol . The effect of cholesterol on the intracellular level of c-fos was measured by means of immunohistochemistry stain. RESULTS : The incorporation of 3H-TdR of rat cardiac fibroblasts of the middle concentration group (0. 5 g/ L) significantly increased ( P < 0. 05 vs control group) . It was indicated in FCM that the number of rat cardiac fibroblasts in S2phase increased significantly , however , CL of high concentratin(1. 2 g/ L) inhibited the proliferation of rat cardiac fibroblasts with the incorporation of 3H-TdR decreased ( P < 0. 05 vs control group) . CL of middle concentration(0. 5 g/ L) significantly increased the expression of c-fos in rat cardiac fibroblasts ( P < 0. 05 , vs control group) . CONCLUSION: CL of middle concentration can promote the proliferation of rat cardiac fibroblasts which is associated with the change in c-fos .

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-3-4
更新日期/Last Update: 2004-09-01