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慢性低氧后钙转运的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第6期
页码:
495-500
栏目:
基础研究
出版日期:
2004-11-01

文章信息/Info

Title:
The changes of calcium homeostasis in rat cardiomyocytes following chronic hypoxia
作者:
裴建明; 王跃民; 孙　新; 张晓东; 毕　辉; 槐　勇; 张　鹏; 朱妙章
第四军医大学生理学教研室,陕西西安710032
Author(s):
PEI Jian-ming ;  WA N G Yao-min ;  SUN Xin ;  ZHANG Xiao-dong ;  BI Hui ;  HUAI Yong ;  ZHANG Peng ;  ZHU Miao-zhang
Department of Physiology ,Fourth Military Medical University ,Xi’an ,Shaanxi 710032 ,China
关键词:
细胞内钙; Ca2 + -ATP 酶; ryanodine 受体; 低氧
Keywords:
Int racellular calcium ;  calcium-ATPase ;  ryanodine receptor ;  sodium/ calcium exchange ; chronic hypoxia
分类号:
R331
DOI:
-
文献标识码:
A
摘要:
目的:本文研究大鼠慢性低氧后心肌钙转运的变化。方法:将大鼠放置于10 %氧环境下4 周,分离正常及慢性缺氧大鼠的右心室肌细胞,用光谱荧光法测定电刺激和咖啡因引起的细胞内[Ca2 + ]i瞬变,并测定肌浆网膜上的Ca2 + -ATP 酶(SERCA) 和ryanodine 受体(RyR) 蛋白的水平。结果:低氧后电刺激和咖啡因引起的细胞内[Ca2 + ]i瞬变的幅度降低且时程延长,RyR 水平无明显改变但SERCA 已显著减少。结论:Ca2 + -ATP 酶活性和蛋白水平的降 低是低氧钙转运异常的主要原因。
Abstract:
AIM: To determine Ca2 + handling in the heart s of rat s subjected to chronic hypoxia (CH) . METHODS: Spect rofluoromet ry was used to measure int racellular Ca2 + concent ration ( [ Ca2 + ]i) and its responses to electrical stimulation and caffeine in myocytes from the right ventricles of the rats breathing 10 % oxygen for 28 days and the age-matched control. The protein expression of sarcoplasmic reticulum Ca2 + -ATPase (SERCA) and its ryanodine receptor (RyR) were measured. RESULTS :It was found that the amplitude was decreased and the duration was prolonged for electrically induced calcium transient and caffeine induced calcium transient . Further study showed that SERCA expression was suppressed by CH while RyR expression did not alter. CONCLUSION: The results indicate that the attenuated Ca2 + -ATPase is mainly responsible for impaired Ca2 + handling in CH.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2004-3-3
更新日期/Last Update: 2004-11-01