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猪供心热缺血移植后对细胞凋亡和Bcl2、Bax、Fas表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第1期
页码:
10-12
栏目:
基础研究
出版日期:
2005-01-05

文章信息/Info

Title:
Effects of warm ischemia heart allograft transplantation in pigs on myocardial cell apoptosis and the expressions of Bcl2,Bax and Fas proteins
作者:
孙忠东; 池一凡; 杨铁南; 侯文明; 牛兆倬
青岛大学医学院附属青岛市立医院心脏外科,山东 青岛 266011
Author(s):
SUN Zhongdong; CHI Yifan; YANG Tienan; HOU Wengming; NIU Zhaozhuo
Department of Cardiovascular Surgery, Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao, Shandong 266011, China
关键词:
心脏移植; 热缺血; 细胞凋亡; Bcl2; Bax;  Fas
Keywords:
heart transplantation;  warm ischemia;  apoptosis;  Bcl2; Bax;  Fas proteins
分类号:
R654.2
DOI:
-
文献标识码:
A
摘要:
目的 研究供心常温热缺血后细胞凋亡和Bcl2、Bax、 Fas蛋白表达的变化。方法 利用猪原位心脏移植实验方法，猪18只随机分为3组，对照组(C，n=6)灌注低温保护液后切取供心，4℃保存4 h；热缺血1组（E1，n=6）常温阻断主动脉缺血5 min后，灌注低温保护液后切取供心；热缺血2组（E2，n=6）常温阻断主动脉缺血10 min后，灌注低温保护液后切取供心。3组低温保存供心4 h后分别行原位心脏移植。供心移植2 h后取左心室心肌检测心肌细胞凋亡和Bcl2、Bax、Fas蛋白的表达。结果 供心移植2 h后，E1、E2组心肌细胞凋亡显著高于C组（P<0.01），E2组心肌细胞凋亡显著高于E1组（P<0.01），Bcl2蛋白表达C组高于E1、E2组（P<0.01），Bax和Fas 蛋白表达C组低于E1、E2组（P<0.01）。结论 供心热缺血移植后的心肌细胞凋亡增加，Bcl2、Bax、Fas参与了凋亡发生。
Abstract:
AIM To investigate changes in the expressions of Bcl2,Bax and Fas proteins caused by the warm ischemia heart donor. METHODS The experimental models of pig orthotopic heart transplantation were establisted. 18 pigs were randomly divided into the control group (C), warm ischemia group 1 (E1) and warm ischemia group 2 (E2). In group C,the donor hearts were removed after perfused hypothermia protection and preserved 4 h at 4℃. In group E1, the donor hearts were removed 5 min after aortic clamping and preserved 4 h at 4℃. In groups E2,the donor hearts were removed 10 min after aortic clamping and preserved 4 h at 4℃. Then the donor hearts were transplanted. The terminal transferase UTP nick end labeling (TUNEL), and the expressions of Bcl2,Bax, Fas proteins were detected. RESULTS The myocardial apoptosis index and expressions of Bax,Fas proteins significantly increased,and the expression of Bcl2 proteins significantly decreased in groups E1 and E2 compared with group C. CONCLUSION This study demonstrated that the donor heart warm ischemia increase myocardial cell apoptosis and influence the expressions of Bcl2,Bax, and Bas proteins.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2004-04-20.基金项目：青岛市科委资助项目（2001KNS2E51）
更新日期/Last Update: 2010-01-05