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Title:: Acute isolation and electrophysiological recording of rabbit ventricular myocytes

作者:: 王军奎¹; 崔长琮²; 官功昌¹; 李宏波¹; 冯新正³; 万田郎³; 1. 陕西省人民医院心内科，陕西西安 710068；2. 西安交通大学第一医院心内科；3. 西安交通大学生物医学研究中心

Author(s):: WANG Junkui¹; CUI Changcong²; GUAN Gongchang¹; LI Hongbo¹; FENG Xinzheng³; WAN Tianlang³; 1. Department of Cardiology , Shaanxi Provincial People′s Hospital, Xi'an , Shaanxi 710068, China

关键词:: 兔; 细胞分离; 离子通道

Keywords:: rabbit; myocyte isolation; ionic channel

分类号:: R331

DOI:: -

文献标识码:: A

摘要:: 目的建立一种简单、稳定的兔心室肌细胞分离方法并进行多种电生理记录。方法采用Langendorff灌流装置及急性酶解分离技术获取单个兔心室肌细胞，并利用全细胞膜片钳技术记录动作电位及多种膜电流。结果耐钙心室肌细胞的存活率在50%～60%，其静息膜电位在-80～90 mV，并成功地记录到典型的动作电位及钾、钠、钙等电流。结论该方法简便、稳定，细胞存活率高且易于进行各种电生理实验。

Abstract:: AIM To construct a simple and stable method of isolating single ventricular myocytes for various electrophysiological recordings. METHODS The single ventricular myocytes of rabbit were isolated enzymatically by modified Langendorff perfusion technique, and action potential and membrane current were recorded by using the wholecell patch clamp technique. RESULTS The survival rate of calciumtolerant myocytes was 50%~60%, and their rest potentials were between –80～90 mV. Typical figures of action potential and natrium, potassium and calcium currents were recorded successfully in single myocytes. CONCLUSION This method is simple and stable and myocytes obtained by the method are well suited for electrophysiological experiments.

参考文献/References

［1］商立军，臧益民，王四旺. 耐钙心肌细胞的分离及基本电生理特性［J］ . 第四军医大学学报，2000，21（2）：247-249.

［2］ Grinwald PM, Nayler WG. Calcium entry in the calcium paradox［J］. J Mol Cell Cardiol, 1981, 13(10):867-880.

［3］陈吉球.心肌细胞分离术［J］. 中国病理生理杂志，1999，15（5）：475-477.

［4］曹春梅，张雄，陈莹莹，等. 不同用途心室肌细胞的分离［J］. 浙江大学学报（医学版），2003，32（1）：51-55.

［5］裴建明，臧益民，牛国保，等. 心肌细胞的分离与保存［J］. 心功能杂志，1996，8（1）：53-54.

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兔心室肌细胞的急性分离及电生理记录(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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