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大剂量硝酸甘油加重大鼠离体心脏缺血/再灌注损伤(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第3期
页码:
204-206
栏目:
基础研究
出版日期:
2005-05-05

文章信息/Info

Title:
A large dose of nitroglycerin aggravates myocardial reperfusion injury in the isolated perfused rat heart
作者:
范谦1;  王文勇2;  高峰1;  马新亮1
第四军医大学: 1.生理学教研室,2.病理学教研室，陕西 西安 10032
Author(s):
FAN Qian1;  WANG Wenyong2;  GAO Feng1;  MA Xinliang1
1. Department of Physiology, Fourth Military Medical University, 2. Department of Pathology2, Fourth Military Medical University, Xi'an,Shaanxi 710032, China
关键词:
心肌缺血/再灌注损伤; 硝酸甘油; 凋亡
Keywords:
myocardial ischemia reperfusion injury;  nitroglycerin;  apoptosis
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 研究大剂量硝酸甘油（NG）对心肌缺血再灌注（MI/R）损伤的影响。方法 SD大鼠30只，制备离体MI/R模型，并将其随机分为2组：NG组和对照组。再灌注开始时NG组给予硝酸甘油（15 μg/h, 溶剂为950 ml/L乙醇, 加药速度50 μl/h），对照组给予950 ml/L乙醇(50 μl/h)，观察心功能指标，检测冠脉流出液中的肌酸激酶（CK）和乳酸脱氢酶（LDH）含量，用伊文氏兰－红四氮唑（TTC）染色和TUNAL法观测再灌注心肌坏死和凋亡程度。结果 大剂量NG抑制了再灌注后心功能的恢复，引起CK、LDH释放增加，促进心肌细胞坏死和凋亡。结论 大剂量NG加重了大鼠离体MI/R损伤。
Abstract:
AIM This study examined the effects of nitroglycerin on cardiac function and cellular injury following ischemia (30 min) and reperfusion (120 min) in isolated perfused rat hearts. METHODS A large dose of nitroglycerin (nitroglycerin treated group, 15 μg/h) or 950 ml/L ethanol（vehicle, 50 μl/h）was administered at the time of reperfusion. RESULTS Administration of a large dose of nitroglycerin significantly aggravated postischemic myocardial injury characterized by depressed cardiac function recovery (P<0.05 vs vehicle), increased lactic dehydrodenase (LDH) （nitroglycerin vs vehicle: 20.3% vs 42.2%, P<0.01）and creatine kinase (CK) release (P<0.01 vs vehicle), enlarged cardiomyocyte necrosis （nitroglycerin vs vehicle: 20.3% vs 42.2%, P<0.01) and apoptosis (nitroglycerin vs vehicle: 30.2% vs 5.2 0.8, P<0.01). CONCLUSION These results suggest that a large dose of nitroglycerin may aggravate MI/R injury.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2004-06-15.
更新日期/Last Update: 2010-01-05