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尼氟灭酸对大鼠心室肌细胞钠通道及动作电位的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第3期
页码:
201-203,206
栏目:
基础研究
出版日期:
2005-05-05

文章信息/Info

Title:
Effects of niflumic acid on the current of Na+ channel and the action potential in islated rat cardiac ventricular myocytes
作者:
杨俊1; 陈艳明1; 王跃民1; 赖小刚1; 周士胜2
1．第四军医大学生理学教研室，陕西 西安 710032；2．大连大学医学院生理学教研室，辽宁 大连 116622
Author(s):
YANG Jun1; CHEN Yanming1; WANG Yuemin1;  LAI Xiaogang1; ZHOU Shisheng2
1. Department of Physiology, Fourth Military Medical University，Xi’an，Shaanxi 710032，China; 2. Department of Physiology, Medical School of Dalian University, Dalian, Liaoning 116622, China
关键词:
阴离子通道; 动作电位; 钠电流; 心脏; 阴离子通道阻断剂
Keywords:
anion channel; action potential; sodium current; heart; anion channel blockers
分类号:
R331.38
DOI:
-
文献标识码:
A
摘要:
目的 观察尼氟灭酸（niflumic acid，NFA）对大鼠心室肌细胞钠电流及动作电位的影响。方法 分别用全细胞膜片钳及电流钳技术记录单个心室肌细胞电压门控钠电流(INa)和动作电位(AP)。结果 尼氟灭酸（100 μmol/L）能可逆性地抑制INa和AP，与对照组比较，抑制的程度分别为：60.1%±12.1% （-30 mV，n=6，P<0.01）及78.2%±10.3%（n=5，P<0.01）。天冬氨酸根离子（Asp–）替代细胞外液的氯离子可引起类似尼氟灭酸的作用。结论 尼氟灭酸对钠通道有抑制作用并影响动作电位。
Abstract:
AIM To study the effects of niflumic acid on the current of Na+ channel and action potential in islated rat cardiac ventricular myocytes. METHODS Wholecell patchclamp and currentclamp techniques were used to measure the voltagegated Na+ current (INa) and action potential (AP) respectively in single rat cardiac ventricular myocytes. RESULTS Niflumic acid (NFA), an anion channel blocker, reversibly inhibited INa and AP. In comparison with the control, NFA decreased INa and AP amplitude (APA) by 60.1%±12.1% (-30 mV，n=6, P<0.01) and 78.2%±10.3%(n=5, P<0.01) respectively. The inhibitory effects of NFA on INa and AP were mimicked by replacing the extracellular Cl- with less permeable anion, aspartate (Asp-). CONCLUSION These data suggested that niflumic acid can inhibit Na+ channel, and influence action potential.

参考文献/References

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［6］ 李耀秦，项红雨，王跃民，等. 氯通道阻断剂对心室收缩和乌头碱诱发性心律失常的影响［J］。心脏杂志，2003，15（3）：250-253.

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备注/Memo

备注/Memo:
收稿日期：2004-06-14.基金项目：国家自然科学基金资助项目（30270602）
更新日期/Last Update: 2010-01-05