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代谢抑制处理后大鼠心室肌细胞反向Na+/Ca2+交换体转运功能的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第5期
页码:
413-415,423
栏目:
基础研究
出版日期:
2005-10-05

文章信息/Info

Title:
Changes of the reverse mode of Na+/Ca2+ exchanger in isolated rat ventricular myocytes subjected to metabilic inhibition
作者:
王波2;  李树壮1;  韦耿泽1;  金振晓1;  胡玉珍1;  周京军1;  张海锋1;  何 炜4
第四军医大学：1.基础部生理学教研室，3.西京医院心脏外科，4.药学系化学教研室，陕西 西安 710032；2.陕西师范大学生命科学学院，陕西 西安 710062
Author(s):
WANG Bo2;  LI Shuzhuang1;  WEI Gengze1;  JIN Zhenxiao3;  HU Yuzhen1; ZHOU Jingjun1;  ZHANG Haifeng1;  HE Wei4
Fourth Military Medical University: 1. Department of Physiology, 3. Department of Cardiology, Xijing Hospital, 4. Department of Chemistry，Shaanxi Xi’an 710032; 2. College of Life Sciences, Shaanxi Normal University, Shaanxi Xi’an 710062
关键词:
钠/钙交换体; 心肌细胞; Ca2+测定; 代谢抑制
Keywords:
Na+/Ca2+ exchange;  ventricular myocytes;  calcium;  metabolic inhibition
分类号:
Q463
DOI:
-
文献标识码:
A
摘要:
目的 利用荧光标记法观察代谢抑制处理后，大鼠心肌细胞反向Na+/Ca2+交换体（NCX）转运功能的变化。方法 酶解法分离制备钙耐受心肌细胞用Fura2/AM负载，采用双激发荧光光电倍增系统（IonOptix Photometry System）检测钙信号。结果 细胞置于无Na+液后，可见［Ca2+］i逐渐升高，L型Ca2+通道阻断剂nifedipine在浓度为1 μmol/L时，不影响此现象；而NCX的抑制剂Ni2+，在浓度为1 mmol/L时，则完全阻断［Ca2+］i的升高。采用20 mmol/L乳酸加10 mmol/L脱氧葡萄糖作为代谢抑制物处理心肌细胞不同时间，正常Tyrode液灌流10 min，之后检测无Na+液引发［Ca2+］i升高效应的变化，发现5 min处理与对照组无显著性差异，10和30 min处理后此效应逐渐减弱。结论 首次发现，代谢抑制处理后心肌NCX的反向转运功能被抑制，阐明其调节机制，将为心肌缺血/再灌注损伤的治疗提供新思路。
Abstract:
AIM To study the reverse mode of Na+/Ca2+ exchanger (NCX) in isolated adult rat ventricular myocytes subjected to metabolic inhibition. METHODS Single left ventricular myocytes were enzymatically isolated and loaded with Fura2/AM. Intracellular calcium concentration (［Ca2+］i) was measured by IonOptix Photometry System. RESULTS ① ［Ca2+］i was increased after ventricular myocytes exposed to Na+free solution (NMDG solution), and returned to baseline following washout. Nifedipine (1 μmol/L), the selective inhibitor of Ltype calcium channel, did not affect NMDG solutioninduced increases in ［Ca2+］i, while Ni2+ (1 mmol/L), the selective inhibitor of NCX, almost completely blocked the effect. ②In myocyte subjected to 20 mmol/L lactate and 10 mmol/L Deoxyglucose treatment for 5, 10 and 30 min respectively, followed by 10 min normal Tyrode solution perfusion, NMDG solutioninduced increases of ［Ca2+］i was timedependently inhibited. CONCLUSION The findings provide evidence that after metabolic inhibition treatment the reverse mode of NCX in ventricular myocytes is attenuated.

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备注/Memo

备注/Memo:
收稿日期：2005-01-19.基金项目：国家自然科学基金（No.30400177)
更新日期/Last Update: 2010-01-05