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Tamoxifen对人心房肌细胞超快速激活延迟整流钾电流的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第6期
页码:
508-510
栏目:
基础研究
出版日期:
2005-12-05

文章信息/Info

Title:
Effects of Tamoxifen on ultrarapid activated delayed rectifier K+ current in human atrial myocytes
作者:
彭文蔚1;  高 瞻2;  朱妙章2;  周京军2; 朱肖星2; 裴兆辉2
1. 江西中医药高等专科学校外科学教研室,江西 抚州 344000；2. 第四军医大学生理学教研室, 陕西 西安 710032
Author(s):
PENG Wenwei1;  GAO Zhan2;  ZHU Miaozhang2;  ZHOU Jingjun2;  ZHU Xiaoxing2;  PEI Zhaohui2
1. Department of Surgery, Jiangxi Traditional Chinese Medicine College, Fuzhou,Jiangxi 344000, China; 2. Department of Physiology, Fourth Military Medical University, Xi′an,Shaanxi 710032,China
关键词:
超快速激活; 延迟整流钾电流; 人心房肌细胞; 膜片钳
Keywords:
tamoxifen;  ultrarapid activated delayed rectifier K+ current;  human atrial cardiomyocyte;  patch clamp
分类号:
R331.31
DOI:
-
文献标识码:
A
摘要:
目的 研究发现tamoxifen可延长患者心电图QT间期，易致心律失常，但其离子机制目前还不清楚。本实验将观察tamoxifen对人心房肌细胞超快速激活延迟整流钾电流(IKur)的影响，以探讨tamoxifen影响心肌QT间期的可能机制。方法 通过胶原酶消化法得到单个人心房肌细胞，并通过全细胞电压钳方法记录心肌细胞上的IKur。结果 本法可得到横纹清楚的单个耐钙人心房肌细胞，Tamoxifen(1~30 μmol/L)可剂量依赖性并可逆地抑制IKur 。结论 Tamoxifen对人心房肌细胞IKur具有显著的抑制作用。
Abstract:
AIM To Pervious studies had shown that tamoxifen altered the QT intervals of patients in clinics, but its ionic mechanism is not clear. The present study was to investigate the effects of tamoxifen on ultrarapid delayed rectifier K+ curent (IKur) in human atrial myocytes. METHODS Single human atrial myocytes were obtained enzymetically and IKur was recorded with wholecell patchclamp techniques. RESULTS The single human atrial cardiomyocytes with clear striation were obtained by the present methods, IKur was inhibited by tamoxifen (1-30 μmol/L) dosedependently and reversibly. CONCLUSION Tamoxifen exerts inhibitory effect on IKur in human atrial myocytes.

参考文献/References

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［3］ Gao Z, Lau CP, Chiu SW, et al. Inhibition of ultrarapid delayed rectifier K+ current by verapamil in human atrial myocytes ［J］. J Mol Cell Cardiol, 2004, 36(2): 257-263.

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备注/Memo

备注/Memo:
收稿日期：2004-08-06.通讯作者：高瞻，讲师，博士，主要从事心脏电生理的研究Tel：(029)83374521Email：zhangao@fmmu.edu.cn作者简介：彭文蔚，讲师Email.jxxuzeyu@163.com
更新日期/Last Update: 2010-01-06