比索洛尔对高血压大鼠心肌成纤维细胞增殖及胶原合成的影响(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2005年第6期
- 页码:
- 511-513
- 栏目:
- 基础研究
- 出版日期:
- 2005-12-05
文章信息/Info
- Title:
- Effects of Bisoprolol on proliferation and collagen synthesis of cultured cardiac fibroblasts from SHR
- Author(s):
- ZHANG Haitao1; ZHAO Lianyou2; LIU Chaozhong1; LUO Huilan1; Sun Jinjin1; YU Xinya1
- 1. Department of Cardiology,General Hospital of Air Force, PLA, Beijing 100036, China; 2.Department of Cardiology ,Tangdu Hospital of Fourth Military Medical University,Xi′an，Shaanxi 710038, China
- Keywords:
- fibroblasts; cardiac myocyte; bisoprolol; proliferation; collage synthesis; hypertension; rat
- 分类号:
- R541.3;Q253
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 探讨比索洛尔对培养的高血压大鼠（SHR）和Wistar大鼠心脏成纤维细胞（CFs）增殖及胶原合成的影响。方法 采用胰酶消化法培养CFs，采用3H胸腺嘧啶核苷（3HTdR）掺入法测定CFs的DNA合成功能，四氮唑蓝（MTT）比色法测定细胞增殖，3H脯氨酸（3HProline）掺入法测定胶原合成。结果 ①基础状态下，SHR组CFs的3HProline掺入量、3HTdR掺入量及MTT比色法A值均明显高于Wistar组。②不同浓度比索洛尔对两组大鼠CFs3HTdR掺入量及MTT比色法A值均无明显作用。③不同浓度比索洛尔以浓度依赖的方式抑制CFs 3HProline掺入量，与Wistar大鼠组相比，SHR组CFs受抑制的效应更强。结论 SHR的CFs细胞数目、DNA合成及胶原含量均存在异常，比索洛尔呈浓度依赖性抑制CFs的胶原合成，但对CFs增殖及DNA合成无明显作用。
- Abstract:
- AIM To evaluate the effects of bisoprolol on proliferation and collage synthesis of cultured rat cardiac fibroblast (CFs) derived from SHR and Wistar rat. METHODS CFs derived from 12weekold SHRs and Wistar rats were cultured with enzymatic dissociation. DNA synthesis was measured by,3HTdR incorporation. Cell numbers were determined by modified MTT array， 3Hproline incorporation was adopted to determine collagen synthesis. RESULTS ①Under basal condition，the incorporation of 3HProline and 3HTdR and A value in CFs derived from SHR were obviously greater than those of Wistar rats. ②Treatment with 0.01-10 μmol/L bisoprolol for 72 h did not produce significant differences of 3HTdR incorporation and A value in SHRs and wistar rats,compared with the control (P>0.05). ③ With the increase of concentration in the effects on bisoprolol, 3HProline incorporation in CFs of two groups decreased. CONCLUSION Bisoprolol inhibits collagen synthesis of CFs in a dosedependent manner. But it does not exert any effects on proliferation and DNA synthesis.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2004-07-28.作者简介：张海涛，主治医师，硕士Tel：（010）80682773Email：kjzht@sina.com
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