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慢性缺氧对大鼠血浆内皮素及心肌ETRa、CaM和CaMKII的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第6期
页码:
514-516
栏目:
基础研究
出版日期:
2005-12-05

文章信息/Info

Title:
Impact of chronic hypoxia on plasma endothelin and ETRa,CaM,CaMKII in cardiac cells
作者:
赵鹏军; 孙锟; 潘俊; 朱飞云;  钱龙华
上海交通大学医学院附属新华医院，儿童医学中心， 上海 20009
Author(s):
ZHAO Pengjun;  SUN Kun;  PAN Jun;  ZHU Feiyun;  QIAN Longhua
Department of Children′s Cardiology,Xinhua Hosptail,SSMU, Shanghai Children's Medical Center,Shanghai 200092,China
关键词:
慢性缺氧;  内皮素;  钙调素;  钙/钙调素依赖性蛋白激酶II
Keywords:
chronic hypoxia;  endothelin;  calmodulin;  CaMKII
分类号:
R542.2；R331.31
DOI:
-
文献标识码:
A
摘要:
目的 研究慢性缺氧对大鼠血浆内皮素和心肌酶的影响。方法 以常压缺氧方式建立缺氧动物模型，SD幼鼠被随机分为3组：正常组、缺氧1周组和缺氧3周组，通过酶联免疫吸附试验检测不同组动物血浆内皮素变化，通过RTPCR方法检测心肌组织中ETRa、CaM、CaMKII的mRNA表达。结果 血浆内皮素水平随缺氧时间延长而升高，正常组、缺氧1周组和缺氧3周组比较均有差异（P<0.05）。同时，心肌细胞ETRa、CaM、CaMKII mRNA的转录水平也随缺氧时间延长而增加，缺氧1周组和正常组比较有差异（P<0.05）；但缺氧3周组和缺氧1周组比较有所不同，CaM和CaMKIIδ转录水平表现有差异（P<0.05），而ETRa 和CaMKIIγ转录水平无差异（P>0.05）。 结论 慢性缺氧使动物血浆中内皮素水平升高，心肌细胞ETRa、CaM和CaMKII mRNA转录增加，可能会对心脏功能产生影响。
Abstract:
AIM To investigate the impact of chronic hypoxia on the changes of plasma endothelin concentration and myocardial enzymes in rats. METHODS A hypoxia model was established on SD rats under normal air pressure with FiO2: 10%. The rats were randomly divided into three groups: the normal group, the group with 1 week, and the group with 3 week hypoxia. The levels of plasma endothelin were measured using ELISA method and the transcription of mRNA of ETRa, CaM and CaMKII were tested by RTPCR. RESULTS Hypoxia elevated the levels of plasma endothelin in a timedependent manner with significant differences among three groups (P<0.05). The transcriptions of mRNA of ETRa, CaM, and CaMKII were also significantly upregulated in 1 week group (P<0.05) and 3 week group. Between the two groups; the transcription of mRNA of CaM and CaMKIIδ were significant different (P<0.05), while ETRa and CaMKIIγ were unchanged (P>0.05). CONCLUSION The hypoxia induced elevation of plasma endothelin and upregulation of the transcription of mRNA of ETRa, CaM and CaMKII may exert some impacts on cardiac functions.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2004-08-05.作者简介：赵鹏军，主治医师，博士生Tel：(021)65793756 Email：pjunzhao@sina.com
更新日期/Last Update: 2010-01-06