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同型半胱氨酸对血管内皮细胞E-选择素、IL-6分泌的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第1期
页码:
57-59
栏目:
基础研究
出版日期:
2006-01-01

文章信息/Info

Title:
Homocysteine activates the expression of E-selectin and interleukin-6 in cultured vascular endothelial cells
作者:
杨大春;  周兴文;  速晓华;  张继红;  杨永健;  陈劲松
成都军区总医院心内科,四川 成都 610083
Author(s):
YANG Da-chun;  ZHOU Xing-wen;  SU Xiao-hua;  ZHANG Ji-hong;  YANG Yong-jian;  CHEN Jin-song
Department of Cardiology, General Hospital of Chengdu Army, Chengdu,Sichuan 610083,China
关键词:
同型半胱氨酸; 内皮细胞; E-选择素; 白细胞介素-6; 动脉粥样硬化
Keywords:
homocysteine; endothelial cells; E-selectin; interleukin-6; atherosclerosis
分类号:
R331.34
DOI:
-
文献标识码:
A
摘要:
目的 探讨同型半胱氨酸（homocysteine ，Hcy）对人脐静脉内皮细胞E-选择素、白细胞介素-6（IL-6）分泌的影响。方法 在人脐静脉内皮细胞ECV304培养基中加入不同浓度Hcy，孵育不同时间，用ELISA法分别测定培养上清液中E-选择素及IL-6的含量。结果 终浓度为250 μmol/L 的Hcy孵育内皮细胞，E-选择素的分泌在4 h显著上调，6 h达峰值；IL-6的分泌在6 h开始升高，24 h达峰值,之后随着时间的延长逐渐下降。Hcy呈浓度依赖性（50 μmol/L～500 μmol/L）促进内皮细胞E选择素及IL-6的分泌。结论 Hcy能刺激血管内皮细胞分泌E-选择素及IL-6。
Abstract:
AIM To investigate the effect of homocysteine (Hcy)on expression of Eselectin and interleukin6 (IL6) in cultured human umbilical vein endothelial cells (HUVEC). METHODS Cultured HUVECs were stimulated with Hcy of different concentrations for different lengths of time. The supernatants were extracted for measurement of secretion of E-selectin and IL6 with ELISA. RESULTS The expression processes of the Eselectin and IL-6 varied following the treatment with 250 μmol/L Hcy. Eselectin expression increased at 4 h and reached the peak at 6 h. Il-6 expression reached the peak at 24 h and then descended gradually. Hcy significantly increased E-selectin and IL6 expression in HUVECs in a concentrationdependent manner. CONCLUSION Hcy may promote the inflammation process by activating E-selectin and IL-6.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-03-30.作者简介：杨大春，主治医师，硕士 Tel：（028）86570341 Email：Yangdc71@163.com
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