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成熟心耳肌细胞自体移植的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第2期
页码:
143-145
栏目:
基础研究
出版日期:
2006-03-01

文章信息/Info

Title:
Experimental study of autologous mature auricle cardiomyocyte transplantation
作者:
张浩; 蔡振杰; 俞世强; 赵璧君; 张近宝
第四军医大学西京医院心血管外科中心,陕西 西安710032
Author(s):
ZHANG Hao;  CAI Zhen-jie;  YU Shi-qiang;  ZHAO Bi-jun;  ZHANG Jin-bao
Center of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi′an，Shaanxi 710032, China
关键词:
心肌细胞学; 移植; 心肌梗塞
Keywords:
myocardium cytology; transplantation;  myocardial infarction
分类号:
Q255;R542.2
DOI:
-
文献标识码:
A
摘要:
目的 将急性分离的成年兔自体左心耳心肌细胞移植到自体梗死区心肌后，研究其存活情况，以及对心律的影响。方法 结扎成年兔的冠状动脉前降支，建立心肌梗死模型，4周后获取自体左心耳组织，急性消化分离为单细胞，经DAPI标记后，分别将细胞悬液和培养基注射到移植组和对照组梗死区内。4周后行心电图检查并取移植区组织进行组织学观察。结果 4周后移植组与对照组兔全部存活。心电图检查，移植组心率略高于对照组（P<0.05），未见异位心律。心肌组织切片见对照组梗死区内为典型心肌梗死后改变，移植组梗死区内有“细胞岛”形成，荧光检测证明移植的心耳肌细胞在移植区存活。结论 急性分离的自体左心耳心肌细胞移植到梗死区心肌内可以存活，并不产生异位心律。
Abstract:
AIM To investigate the survival of grafted autologous left auricle cardiomyocytes and the effect on heart rate. METHODS MI models were established by ligating the left anterior descending artery. Four weeks later the left auricle was harvested and the auricle heart cells were isolated and labeled with DAPI ex vivo. Either a cell suspension (transplant group) or culture medium (control group) was injected into the infarcted areas. Electrocardiogram (ECG) was conducted after 4 weeks and the specimens were harvested and observed by histologic methods. RESULTS The rabbits all survived four weeks after injection. ECG showed that the heart rate in the transplant group was slightly faster than that in control group (P<0.05). No ectopic rhythm was observed. The infarcted areas from the sacrificed rabbits revealed typical changes of myocardial infarction in control group and "cell island" was observed in transplant group. Fluorescence detection confirmed the survival of implanted cells. CONCLUSION Isolated autologous left auricle cardiomyocytes can survive after grafted into rabbit ventricular myocardium following myocardial infarction and will not induce ectopic rhythm.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-09-20.通讯作者：蔡振杰，主任医师，教授，主要从事冠心病临床方面的研究 Tel：(029) 84775314 Email：Caizhenjie@hotmail.com 作者简介：张浩，硕士生 Tel: (029) 84775314 Email:zhanghaoo@hotmail.com
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