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NF-κB在TNF-α致人脐静脉内皮细胞凋亡中的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第2期
页码:
152-154
栏目:
基础研究
出版日期:
2006-03-01

文章信息/Info

Title:
Role of NF-κB in endothelial cell apoptosis induced by TNF-α
作者:
郭瑞威; 杨丽霞; 石燕昆; 齐峰; 郭传明
成都军区昆明总医院心血管内科，云南 昆明 650032
Author(s):
GUO Rui-wei;  YANG Li-xia;  SHI Yan-kun;  QI Feng;  GUO Chuan-ming
Department of Cardiology, Kunming General Hospital, Chengdu Military Area Command, Kunming,Yunnan 650032, China
关键词:
核转录因子-κB; 内皮细胞; 肿瘤坏死因子-α; 凋亡
Keywords:
nulear factor-κB; endothelial cells; tumor necrosis factor-α; apoptosis
分类号:
R329.28
DOI:
-
文献标识码:
A
摘要:
目的 研究核转录因子-κB （nuclear factor-κB,NF-κB）在TNF-α诱导培养的人脐静脉内皮细胞凋亡过程中的作用。方法体外培养人脐静脉内皮细胞，并用10 ng/ml的TNF-α进行诱导，不同时间段观察NF-κB活性、NF-κB抑制物IκBα表达以及细胞凋亡的情况，EMSA测定NF-κB活性，Western-blot检测IκBα的表达情况；Tunel法检测细胞凋亡；并用NF-κB 的抑制剂PDTC预处理细胞后来观察TNF-α诱导细胞凋亡的情况。结果TNF-α以时间依赖性诱导人脐静脉内皮细胞凋亡，NF-κB的活性在处理后10 min开始增强，2 h后恢复正常，IκBα的表达在10 min开始下降，2 h恢复正常；PDTC能抑制TNF-α诱导的凋亡。结论TNF-α在诱导体外培养的人脐静脉内皮细胞时，IκBα降解，NF-κB激活，从而引起细胞的凋亡。
Abstract:
AIM To study the role of nulear factor-κB (NF-κB) in endothelial cell apoptosis induced by TNFα. METHODS The cultured endothelial cells were treated with 10 ng/ml TNF-α in absence and presence of PDTC (an inhibitor of NF-κB), the activity of NF-κB was measured by e1lectrophoretic mobility shift assay (EMSA), the expression of IκBα was analyzed by western blotting and apoptosis of cells was evaluated by TUNEL. RESULTS TNF-α induced apoptosis of endothelial cells in a timedependant manner. The activity of NF-κB began to increase and the expression of IκBα began to decrease at 10 min after treatment with TNF-α. PDTC inhibited the apoptosis induced by TNF-α. CONCLUSION TNF-α activates NF-κB through phosphorylation of IκBα and thus induces apoptosis of endothelial cells.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-09-16.基金项目：云南省科委自然科学基金项目资助（No.2001C0070W） 通讯作者：杨丽霞，主任医师，主要从事心力衰竭基础与临床的研究 作者简介：郭瑞威，硕士生 Tel：（0871）4074571 Email：grw771210@163.com
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