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|本期目录/Table of Contents|

低浓度过氧化氢对内皮前体细胞的损伤效应及复方丹参注射液的保护作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第4期
页码:
414-416,421
栏目:
临床研究
出版日期:
2006-08-25

文章信息/Info

Title:
Injury caused by low concentration hydrogen peroxide in cultured endothelium progenitor cells and protective effect of injectio salviae miltiorrhizae
作者:
周祥; 王海昌; 宗小娟; 程康
第四军医大学西京医院心内科，陕西 西安 710033
Author(s):
ZHOU Xiang;  WANG Hai-chang;  ZONG Xiao-juan;  CHENG Kang
Department of Cardiovasology, Xijing Hospital, Fourth Military Medical University, Xi′an，Shaanxi 710033, China
关键词:
内皮前体细胞; 过氧化氢; 复方丹参注射液; 损伤
Keywords:
endothelium progenitor cells; hydrogen peroxide; injectio salviae miltiorrhizae; injury
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的 观察低浓度过氧化氢（H2O2）对内皮前体细胞（EPC）的损伤效应及复方丹参注射液（ISM）的保护作用。方法 建立猪EPC体外培养模型，在培养液中加入低浓度H2O2（100、200、300、400 μmol/L）及5 mg/L ISM，测定细胞增殖活力（MTT法）、乳酸脱氢酶 (LDH)活性、丙二醛(MDA)含量及细胞凋亡率。结果 H2O2使细胞增殖活力下降，使 LDH、MDA含量增加，细胞凋亡率上升，并呈浓度依赖性，ISM可减轻上述变化。结论 ISM对低浓度H2O2造成的EPC损伤具保护作用。
Abstract:
AIM To observe the injury caused by low concentration hydrogen peroxide (H2O2) in cultured endothelium progenitor cells (EPCs) and the protective effect of injectio salviae miltiorrhizae (ISM). METHODS EPCs were cultured in vitro and H2O2 of different low concentrations (100, 200, 300, 400μmol/L) and 5 mg/L ISM were added into culture medium. The proliferative activity of cells (MTT assay), the concentration of lactate dehydrogenase (LDH), malondialdehyde (MDA) and the rate of cell apoptosis were assayed. RESULTS Compared with that of the control, H2O2 decreased the proliferative activity but increased MDA, LDH and the rate of cell apoptosis. These results were inhibited by ISM. CONCLUSION ISM can protect EPCs from injury caused by H2O2.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2006-02-21.基金项目：国家自然科学基金项目资助（No.30370581）通讯作者：王海昌，主任医师，主要从事冠心病的基础研究和临床治疗研究 Tel：（029）84775184 作者简介：周祥，住院医生，硕士 Tel：（029）82554079 Email：jixiang@fmmu.edu.cn
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