我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

金黄色葡萄球菌对左心室细胞黏附分子和炎症因子基因表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第5期
页码:
492-495
栏目:
基础研究
出版日期:
2006-10-25

文章信息/Info

Title:
Effect of Staphylococcus aureus on expressions of cell adhesion molecules and inflammatory mediator in left ventricular myocardium
作者:
赵波; 孙威; 付向宁; 陈启福
华中科技大学同济医学院附属同济医院胸心外科，湖北 武汉 430030
Author(s):
ZHAO Bo;  SUN Wei;  FU Xiang-ning;  CHEN Qi-fu
Department of Tharocardiosurgery, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan，Hubei 430030, China
关键词:
心内膜炎; 金黄色葡萄球菌; 黏附分子; 炎症因子
Keywords:
endocarditis;  Staphylococcus aureus;  adhesion molecule;  inflammatory mediator
分类号:
Q256;R542.41
DOI:
-
文献标识码:
A
摘要:
目的 初步探讨金黄色葡萄球菌对左心室细胞黏附分子和炎症因子基因表达的影响和感染性心内膜炎的发病机制。方法 7只健康家兔做对照，24只家兔分别经静脉注射金黄色葡萄球菌（金葡菌）和二尖瓣手术。术后24 h处死，取出左心室组织，肉眼、电镜观察。RT-PCR技术检测细胞黏附分子（E-Selectin, PECAM-1, VCAM-1）和炎症因子巨噬细胞集落刺激因子-1（MCSF-1） mRNA表达水平。结果 单独金葡菌注射没有引起左心室靶基因的显著变化；二尖瓣手术后左心室组织E-Selectin, PECAM-1和MCSF-1 mRNA表达水平显著升高，而VCAM-1 mRNA没有显著的变化；二尖瓣手术并发金葡菌注射后左心室组织E-Selectin和MCSF-1 mRNA表达显著升高，而PECAM-1和VCAM-1 mRNA表达降低。结论 金黄色葡萄球菌在心内膜损伤和病理血流状态下，导致左心室部分特定的细胞黏附分子和炎症因子表达变化，引起感染性心内膜炎发生。
Abstract:
AIM To investigate how Staphylococcus aureus influences gene expressions of cell adhesion molecules (E-Selectin, PECAM-1, VCAM-1) and MCSF-1 in left ventricular myocardium and explore the cellular immunology mechanism of infective endocarditis. METHODS Seven rabbits received 5×106 colony forming units Staphylococcus aureus i.v., 9 rabbits underwent mitral surgery and 8 rabbits underwent both interventions in combination. The hearts were explanted 24 h after the intervention and observed by naked eye and electron microscope. mRNA abundance was determined by semiquantitative duplex RTPCR. Results were compared with those in control group consisting of 7 untreated rabbits. RESULTS Whereas the bacteraemia did not lead to upregulated mRNA levels of any studied molecules, mitral valve surgery caused significantly higher relative expression levels of ESelectin, PECAM-1 and MCSF-1, but not of VCAM-1. The combination of bacteremia and mitral valve surgery caused an uneven expression pattern with a significant upregulation of ESelectin and MCSF-1 and a downregulation of PECAM-1 and VCAM-1. CONCLUSION Staphylococcus aureus leads to changes of regulations of specific cell adhesion molecules and MCSF1 in left ventricular myocardium after surgical procedure, thus resulting in the development of infective endocarditis.

参考文献/References

［1］ Freedman LR. The pathogenesis of infective endocarditis［J］. J Animicrob Chemother, 1987,20 suppl A: 1-6.

［2］Pappa E, Korantzopoulos P, Karanikis P, et al. Combination of traumatic pseudoaneurysm of descending thoracic aorta and bicuspid aortic valve endocarditis in a young adult［J］. Int J Cardiol, 2004,96(2): 287-289.

［3］ Heilmann C, Niemann S, Sinha B, et al. Staphylococcus aureus fibronectin-binding protein (FnBP)mediated adherence to platelets, and aggregation of platelets induced by FnBPA but not by FnBPB［J］. J Infect Dis, 2004,190(2): 321-329.

［4］李棠，周春菊. 感染性心内膜炎的临床病理和病原菌的变化［J］.心肺血管病杂志，1999，18（2）：103-104.

［5］吴丹宁，贾国良. 细胞黏附分子与心肌再灌注损伤［J］.心功能杂志,1997，9（4）：238-241.

［6］Kremlev SG, Chapoval AI, Evans R. CSF1 (MCSF) enhances the inflammatory response of fibronectinprimed macrophages: pathways involved in activation of the cytokine network［J］. Nat Immun, 1998,16(5-6): 228-243.

［7］Sderquist B, Sundqvist KG, Vikerfors T. Adhesion molecules (Eselectin）, intercellular adhesion molecule1 (ICAM1) and vascular cell adhesion molecule1 (VCAM1)) in sera from patients with Staphylococcus aureus bacteraemia with or without endocarditis［J］. Clin Exp Immunol, 1999,118(3): 408-411.

［8］Muller AM, Cronen C, Kupferwasser LI, et al.Expression of endothelial cell adhesion molecules on heart valves: upregulation in degeneration as well as acute endocarditis［J］. J Pathol, 2000,191(1):54-60.

［9］Valen G, Paulsson G, Vaage J. Induction of inflammatory mediators during reperfusion of the human heart［J］. Ann Thorac Surg, 2001,71(1): 226-232.

备注/Memo

备注/Memo:
收稿日期：2005-09-07.通讯作者：赵波、主治医师，主要从事心脏疾病外科治疗研究 Tel：（027）83663408 Email：zhaobo0115@yahoo.com.cn
更新日期/Last Update: