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|本期目录/Table of Contents|

预处理对缺血再灌注时心肌电生理的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第5期
页码:
503-505,512
栏目:
基础研究
出版日期:
2006-10-25

文章信息/Info

Title:
Effect of preconditioning on myocardium electrophysiology during ischemia/reperfusion
作者:
张繁之; 吕家高; 卜军; 王岚; 王琳
华中科技大学同济医学院附属同济医院心内科，湖北 武汉 430030
Author(s):
ZHANG Fan-zhi;  Lv Jia-gao;  BU Jun;  WANG Lan;  WANG Lin
Department of Cardiology, Tongji Hospital, Tongji Medical College, Hua Zhong University of Science and Technology, Wuhan， Hubei 430030,China
关键词:
缺血预适应; 单相动作电位; 有效不应期
Keywords:
ischemic preconditioning;  monophasic action potential;  effective refractory period
分类号:
R331.3;R541.7
DOI:
-
文献标识码:
A
摘要:
目的 探讨缺血预适应(IPC)对缺血/再灌注心律失常的影响及其机制。方法 12只犬随机分为对照组(n=6)和IPC组(n=6)，采用特制电极记录技术，观察和测量三层心肌单相动作电位(MAP) 及有效不应期(ERP)，分析室性心律失常发生率、MAP时程(MAPD)、MAPD跨室壁离散 (TDR)及ERP跨室壁离散(TDE)。结果 对照组6只中有4只发生室速/室颤，而IPC组6只中仅1只发生室速/室颤。对照组缺血时MAPD同步缩短，而ERP延长且三层心肌延长的幅度不一致，再灌注时逐步恢复。IPC组缺血时MAPD缩短与ERP延长均不明显，与对照组相比，具有显著差异(P<0.05)。各组三层心肌之间的MAPD是一致的。对照组缺血/再灌注时ERP明显不一致，TDE增大，而 IPC组TDE小，两者相比较具有显著差异(P<0.05或P<0.01)。 结论 IPC减少缺血/再灌时MAPD、ERP及TDE的变化，这可能是其抗心律失常的部分机制。
Abstract:
AIM To explore the effect and mechanism of which ischemic preconditioning (IPC) protect against arrhythmias induced by ischemia/reperfusion (IR). METHODS Twelvecanines were divided into two groups randomly: control (IR) group and IPC (a single 5 min occlusion and 10 min reperfusion followed by IR) group. With the monophasic action potential (MAP) technique, MAP duration and effective refractory period (ERP) of the three layers myocardium were measured by specially designed plungeneedle electrodes. The incidence of ventricular arrhythmias, transmural dispersion of repolarization (TDR) and transmural dispersion of ERP (TDE) were analyzed. RESULTS In the control group, four of six occurred with ventricular tachycardia/ventricular fibrillation, but one of six occurred with ventricular tachycardia/ventricular fibrillation in IPC group. In the control group, MAP duration shortened similarly, but ERP lengthened in different altitude among the three layers myocardium during ischemia, which was restored during reperfusion. In IPC group, Both MAP duration and ERP did not change obviously, and compared with control group, the difference was statistically significant (P<0.05). Among of the three layers myocardium, MAP duration was uniform in two groups. In control group, compared with IPC group ERP changed obviously and TDE increased during IR (P<0.05 or P<0.01). CONCLUSION IPC reduces both MAP duration shortening and ERP lengthening concomitant with TDE during IR, which may be a part of the electrophysiological mechanism by which IPC protects against the arrhythmias induced by IR.

参考文献/References

［1］ Lawson CS, Coltart DJ, Hearse DJ."Dose"dependency and temporal characteristics of protection by ischaemic preconditioning against ischaemiainduced arrhythmias in rat hearts［J］ . J Mol Cell Cardiol，1993, 25(12):1391-1402.

［2］ Walker MJ, Curtis MJ, Hearse DJ, et al. The lambeth conventions: guidelines for the study of arrhythmias in ischaemia infarction, and reperfusion ［J］. Cardiovasc Res, 1988, 22(7):447-455.

［3］ Taggart P, Sutton PM, Opthof T,et al. Transmural repolarisation in the left ventricle in humans during normoxia and ischaemia［J］. Cardiovasc Res, 2001, 50(3):454-462.

［4］ Zhu J, Ferrier GR.Ischemic preconditioning: antiarrhythmic effects and electrophysiological mechanisms in isolated ventricle ［J］. Am J Physiol, 1998, 274(1 Pt 2):H66-75.

［5］ Shattock MJ, Lawson CS, Hearse DJ, et al. Electrophysiological characteristics of repetitive ischemic preconditioning in the pig heart ［J］. J Mol Cell Cardiol, 1996, 28(6):1339-1347.

［6］ Hamada K, Yamazaki J, Nagao T. Shortening of action potential duration is not prerequisite for cardiac protection by ischemic preconditioning or a KATP channel opener［J］. J Mol Cell Cardiol，1998，30(7):1369-1379.

［7］ 赵正航，臧伟进，张风杰，等. 腺苷对大鼠心肌缺血再灌注心律失常的影响［J］.心脏杂志, 2001,13(4): 251-256.

［8］ Grover GJ, D′Alonzo AJ, Dzwonczyk S,et al. Preconditioning is not abolished by the delayed rectifier K+ blocker dofetilide［J］. Am J Physiol，1996，271(3 Pt 2):H1207-1214.

［9］ 朱妙章,臧益民,贾敏江,等.心肌缺血预适应及其机制［J］.心脏杂志,1999,11(4): 263 - 265.

［10］Hashimoto H, Asano M, Nakashima M. Alternation in refractoriness and in conduction delay in the ischemic myocardium associated with the alternation in the STT complex during acute coronary occlusion in anesthetized dogs［J］. J Electrocardiol,1986，19(1):77-84.

［11］Sutton PM, Taggart P, Opthof T,et al. Repolarization and refractoriness during early ischaemia in humans［J］. Heart，2000，84(4):365-369.

［12］张飞龙，蓝玉福，吴黎明. 缺血预处理对缺血再灌注心室肌有效不应期的影响［J］. 福建医科大学学报，1999，33(2):148-151.

［13］Cascio WE, Yang H, Johnson TA, et al. Electrical Properties and Conduction in Reperfused Papillary Muscle ［J］. Circ Res, 2001, 89(9):807-814.

备注/Memo

备注/Memo:
收稿日期：2005-03-19.通讯作者：吕家高，副主任医师，主要从事心脏电生理研究 Email：lujiagao@tjh.tjmu.edu.cn 作者简介：张繁之，博士生 Tel:(027)83663608 Email：doczfz@hotmail.com
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