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雌激素对PDGF诱导的血管平滑肌细胞增殖及细胞周期的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第6期
页码:
611-613
栏目:
基础研究
出版日期:
2006-12-25

文章信息/Info

Title:
Effect of estrogen on proliferation and cell cycle of cultured rabbit vascular smooth muscle cells induced by platelet-derived growth factor
作者:
周永兰1; 陈清枝2; 张延斌2; 王人彭1; 张义勤1; 骆秉辁1
1．江苏大学附属医院徐州第三人民医院心内科，江苏 徐州 221005；2．徐州医学院附属医院心内科，江苏 徐州 221002
Author(s):
ZHOU Yong-lan1;  CHEN Qing-zhi2;  ZHANG Yan-bin2;  WANG Ren-peng1;  ZHANG Yi-qin1;  LUO Bing-quan1
1.Department of Cardiology, Xuzhou Third People′s Hospital, Affiliated Xuzhou Hospital of Jiangsu University, Xuzhou，Jiangsu 221005； 2. Department of Cardiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou,Jiangsu 221002, China
关键词:
雌二醇; 血管平滑肌细胞; 增殖; 细胞周期; 血小板源生长因子
Keywords:
estradiol; vascular smooth muscle cells;  proliferation;  cell cycle;  platelet-derived growth factor
分类号:
R458.7
DOI:
-
文献标识码:
A
摘要:
目的 探讨17β-雌二醇（E2）对血管平滑肌细胞（VSMC）增殖及细胞周期的影响。方法 分离培养VSMC，血小板源生长因子(PDGF)诱导其增殖，应用MTT法及流式细胞仪检测不同浓度E2（10和100 nmol/L）对传代VSMC增殖活性及细胞周期的影响。结果 E2 (10和100 nmol/ L) 作用下VSMC增殖活性下降, 且与浓度有关；对VSMC细胞周期的分布影响主要表现为处于G0/G1期细胞数增多，G2/S期的细胞数减少（P<0.01）。结论 E2具有抑制VSMC增殖的作用，其部分机制可能是通过阻滞VSMC G0/G1期向S的转化有关。
Abstract:
AIM To investigate the effect of 17β estradiol ( E2) on the proliferation of vascular smooth muscle cells (VSMC) and the possible underlying mechanisms. METHODS A cell proliferating model of rabbit aorta VSMC induced by PDGFBB was established. The proliferation was determined with MTT metabolism in subcultured VSMC exposed to different concentrations （10 and 100 nmol/L） of 17β estradiol and the cell cycle was examined by flow cytometry. RESULTS MTT metabolism of VSMCs induced by PDGFBB was inhibited by E2 and the cells numbers of G0/G1 phase increased and the cells numbers of G2/S phase decreased markedly (P<0.01). CONCLUSION 17β estradiol inhibits the proliferation of VSMC induced by PDGFBB, at least in part by preventing VSMCs entering S phase from G0/G1 phase.

参考文献/References

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［4］ Worboys S,Kotsopoulos D, Teede H,et al．Evidence that parenteral testosterone therapy may improve endotheliumdependent and independent vasodilation in postmenopausal women already receiving estrogen［J］. Clin Endocrinol Metab,2001,86(1): 158-161．

［5］ Sudoh N, Toba K, Akishita M,et al．Estrogen prevents oxidative stress–induced endothelial cell apoptosis in rat［J］. Circulation,2001,103(5):724-729．

［6］ Strehlow K, Rotter S， Wassmann S,et al．Modulation of antioxidant enzyme expression and function by estrogen ［J］. Circ Res,2003,93(2): 170-177．

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备注/Memo

备注/Memo:
收稿日期：2006-01-16.作者简介：周永兰，主治医师，硕士生 Tel：（0516）83770891 Email：zhouyonglan94101@yahoo.com.cn
更新日期/Last Update: