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Title:: Effect of Fosinopril on regulation of Caspase3 in ischemia and reperfusion injury rat myocardium

作者:: 张志国; 秦玲; 赵学忠; 吉林大学第一医院心内科，吉林长春130021

Author(s):: ZHANG Zhi-guo; QIN Ling; ZHAO Xue-zhong; Department of Cardiology, First Hospital of Jilin University, Changchun Jilin 130021, China

关键词:: 心肌缺血; 再灌注损伤; 福辛普利; Caspase-3

Keywords:: myocardial ischemia; reperfusion injury; Fosinpril; Caspase-3

分类号:: R542.2

DOI:: -

文献标识码:: A

摘要:: 目的观察福辛普利对大鼠心肌缺血/再灌注损伤后细胞凋亡关键酶Caspase-3表达的影响。方法结扎大鼠左冠状动脉前降支，30 min后松开结扎线，假手术组只穿线不结扎，大鼠随机分为假手术组、缺血/再灌注组、福辛普利组（20 mg/kg）。福辛普利组分别于术前24 h及术前2 h灌胃给药，观察24 h后心肌细胞电镜下的形态改变和Caspase3 mRNA的表达。结果福辛普利组电镜下肌丝肌节清晰，而缺血再灌注组闰盘结构不清，肌丝断裂，大鼠心肌Caspase-3 mRNA表达灰度值福辛普利组为0.37±0.03，缺血/再灌注组为1.14±0.05(P<0.01)。结论福辛普利可以使心肌缺血/再灌注后的Caspase-3的表达下降。

Abstract:: AIM To study the protective effect of Fosinopril on ischemia and reperfusion rat myocardium and the Caspase3 expression in myocardium. METHODS Wistar rats were randomly divided into three groups: Control group (n=8), IschemiaReperfusion group (n=8), in which the left anterior descending of coronary artery was occluded for 30 min followed by 24 hours reperfusion and Fosinopril (20 mg/kg) group (n=8). Medication was fed through gastric gavage 24 hour and 2 hour before occlusion and the Caspase3 expression in myocardium was observed. RESULTS The expression of Caspase3 mRNA in Fosinpril group was less than that of IschemiaReperfusion group (0.37±0.03 vs 1.14±0.05 and 0.39±0.03 vs 1.14±0.05,P<0.01) respectively. CONCLUSION Fosinpril can reduce Caspase3 expression in myocardium, thus protecting ischemia and reperfusion rat myocardium.

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福辛普利对大鼠缺血/再灌注损伤心肌细胞Caspase3表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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