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白藜芦醇对外周血内皮祖细胞功能的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第6期
页码:
617-621
栏目:
基础研究
出版日期:
2006-12-25

文章信息/Info

Title:
Effects of resveratrol on function of endothelial progenitor cells from peripheral blood
作者:
顾俊1; 王长谦1; 范华骅2; 聂晓绚2; 王彬尧1; 黄定九1
1. 上海交通大学医学院附属仁济医院心内科，上海 200001；2.上海血液中心血液工程研究室，上海 200051
Author(s):
GU Jun1;  WANG Chang-qian1;  FAN Hua-hua2;  NIE Xiao-xuan2;  WANG Bin-yao1;  HUANG Ding-jiu1
1.Department of Cardiology, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200001, China； 2.Laboratory of Blood Engineering, Shanghai Blood Center, Shanghai 200051, China
关键词:
白藜芦醇; 内皮祖细胞; 内皮型一氧化氮合成酶
Keywords:
resveratrol;  endothelial progenitor cells;  eNOS
分类号:
R453;R329.2
DOI:
-
文献标识码:
A
摘要:
目的 观察不同浓度白藜芦醇对于内皮祖细胞体外功能的影响。方法 密度梯度离心法获得人外周血单个核细胞，培养7 d后，采用免疫荧光和流式细胞仪进行细胞鉴定；随后加入不同浓度的白藜芦醇［0(对照)、1、5、15、60 μmol/L］干预72 h，观察其对内皮祖细胞增殖、迁移和黏附能力的影响；同时采用RT-PCR和ELISA方法测定药物干预后，内皮祖细胞中内皮型一氧化氮合成酶（endothelial nitric oxide synthase, eNOS）的表达。 结果 与对照组相比，1 μmol/L白藜芦醇促进内皮祖细胞增殖、迁移和黏附，同时上调eNOS的mRNA和蛋白水平的表达；而60 μmol/L白藜芦醇抑制内皮祖细胞增殖、迁移和黏附，并且下调eNOS的mRNA和蛋白水平的表达。结论 低浓度白藜芦醇可改善内皮祖细胞增殖、迁移和黏附功能，并上调eNOS表达，而高浓度白藜芦醇则抑制上述细胞功能和eNOS表达。
Abstract:
AIM To investigate the effect of resveratrol on the function of endothelial progenitor cells (EPCs). METHODS Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and cultured. After 7 days of culture in vitro, EPCs were identified as adherent cells double positive for DiIacLDL/FITCUEAⅠ under a laser scanning confocal microscope and their expression of CD34, CD31 and KDR was detected by flow cytometry. EPCs were then stimulated with resveratrol (1, 5, 15, 60 μmol/L) or vehicle control for 72 hours. EPCs′ proliferation, migration and adhesion activity were assayed. RT-PCR and ELISA were also performed to determine the eNOS expression after resveratrol interference. RESULTS Incubation of human EPCs with low concentration of resveratrol increased the proliferation, adhesion and migration activity. In addition, low concentration upregulated the eNOS expression. While high concentration of resveratrol inhibited aforementioned functions and eNOS expression. CONCLUSION Low concentration of resveratrol may promote the functional activity and eNOS expression of EPCs, but high concentration has adverse effects.

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备注/Memo

备注/Memo:
收稿日期：2005-12-10.基金项目：国家自然科学基金资助项目（No.30170365） 通讯作者：王长谦，教授，主要从事冠心病介入治疗 Tel:(021)65790000-7050 Email:wcqian@hotmail.com 作者简介：顾俊，硕士生 Tel：（021）64881217-5152 Email：forrestgu@126.com
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