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内皮素1对缺氧/复氧所致心肌细胞凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第1期
页码:
12-15
栏目:
基础研究
出版日期:
2007-01-01

文章信息/Info

Title:
Influences of endothelin1 on apoptosis of hypoxia/reoxygenation myocardiocytes
作者:
郭万刚1 余志斌1 孙立军2 罗中华2
第四军医大学:1.航空航天医学系航空航天生理学教研室,2.西京医院放射科,陕西 西安 710032
Author(s):
GUO Wangang1 YU Zhibin1SUN Lijun2LUO Zhonghua2
1.Department of Aerospace Physiology, School of Aerospace Medicine, 2.Departmdnt of Radiology,Xijing Hospital,Fourth Military Medical University, Xi′an 710032, Shaanxi, China
关键词:
心肌细胞缺氧/复氧细胞凋亡内皮素1
Keywords:
cardiomyocytes hypoxia/reoxyegenation apoptosis endothelin
分类号:
Q255;R331.36
DOI:
-
文献标识码:
A
摘要:
目的 探讨在缺氧/复氧过程中不同时期给予内皮素1(ET1)对缺氧/复氧所致心肌损伤与凋亡的影响。方法 乳鼠心肌细胞缺氧/复氧模型,流式细胞仪检测心肌细胞凋亡,Hoechst 33258染色计算凋亡率,试剂盒检测乳酸脱氢酶(LDH)的活性。结果 ET1预处理组(EP)细胞生存率较缺氧复氧组(HR)提高,凋亡率下降,LDH活性下降;ET1缺氧即刻处理组(EH)细胞生存率较HR组降低,凋亡率升高,LDH释放量增加;ET1复氧处理组(ER)的细胞生存率、凋亡率及LDH活性与HR组均无统计学差异。结论 ET1预处理有心肌细胞保护作用,ET1缺氧时有促进心肌细胞损伤和凋亡的作用。
Abstract:
AIM To explore the effects of endothelin1 at different administrative timing on apoptosis and cell injury induced by hypoxia/reoxygenation. METHODS Primarily cultured neonatal rat cardiomyctes were treated with 950 ml/L N2-50 ml/L CO2 mixture to establish hypoxia/reoxygenation model. Flow cytometry and Hoechst 33258 stain were performed to determine the apoptotic rates of cardiomyocytes. A domestic kit was used to determine the activity of lactate dehydrogenase (LDH). RESULTS Cell viability values, apoptotic rates and lactate dehydrogenase (LDH) activity of cells with 24 h ET1 treatment before hypoxia increased, lowered and decreased respectively compared with those in hypoxia/reoxygenation group (HR). ET1 treatment during hypoxia depressed the cell viability, raised apoptotic rate and LDH release. ET1 treatment during reoxygenation had no effects on these values. CONCLUSIONET1 pretreatment has protective effects on cardiomyocytes from hypoxia/reoxygenation. However, ET1 during hypoxia aggravates the injury of cardiomyocytes induced by hypoxia/reoxygenation.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期:2005-12-26.通讯作者:孙立军,主任医师 Email:slj@fmmu.edu.cn.作者简介:郭万刚,硕士生 Email:guowagon@126.com.
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