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|本期目录/Table of Contents|

心肌细胞内钙释放对心肌细胞PTEN表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第1期
页码:
9-11
栏目:
基础研究
出版日期:
2007-01-01

文章信息/Info

Title:
Effects of calcium release induced by Ryanodine on PTEN expression in cardiomyocytes
作者:
周裔忠1; 2; 祝善俊1; 于林君1; 田颖1; 王江1
1.第三军医大学新桥医院心血管内科，重庆 400037;2.江西省人民医院心内科，江西 南昌 330006
Author(s):
ZHOU Yizhong1; 2;  ZHU Shanjun1;  YU Linjun1;  TIAN Ying1;  WANG Jiang1
Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
钙释放; 第10染色体同源丢失性磷酸酶张力蛋白基因; 负性调控; 心肌细胞
Keywords:
calcium release; phosphatase and tensin homology deleted onchromosome10; negative regulate; cardiomyocyte
分类号:
Q344.13
DOI:
-
文献标识码:
A
摘要:
目的 探讨雷尼丁（Ryanodine，RY）介导的心肌细胞内钙释放对PTEN mRNA 和蛋白表达影响。方法 新生Wistar乳鼠心肌细胞原代培养。用不同浓度的RY（0.01～10 μmol/L）促进心肌细胞内钙释放，特异的钙敏感指示剂 Fura2/AM 测定心肌细胞内钙浓度（［Ca2+］i）。逆转录聚合酶链式反应（RTPCR）、Western blot分别检测PTEN mRNA和蛋白表达。结果 RY在0.01～1 μmol/L浓度范围内，能浓度依赖性地促进心肌细胞内钙释放，RY 在10 μmol/L时，心肌细胞内钙浓度低于RY 在1 μmol/L 时的效应，但仍高于对照组；RY在0.01～1 μmol/L浓度范围内能浓度依赖性促进心肌细胞PTEN mRNA和蛋白表达，RY在10 μmol/时，心肌细胞PTEN mRNA和蛋白表达低于1 μmol/L的效应，但仍高于对照组。结论 RY介导的心肌细胞内钙释放可影响心肌细胞PTEN基因表达，其表达在RY处于0.01～1 μmol/L范围内具有浓度依赖性。
Abstract:
AIM To investigate the effects of calcium release induced by Ryanodine (RY）on phosphatase and tensin homology deleted onchromosome10 (PTEN) expression. METHODS Cardiomyocytes were cultured in Wistar rats aged 13 days. Intracellular calcium concentration (［Ca2+］i) was measured by Fura2/AM, and PTEN mRNA and protein expression was detected respectively by RTPCR and Western blot. RESULTS RY increased ［Ca2+］i of cardiomyocytes, and the PTEN mRNA and protein expression increased with the increase in ［Ca2+］i of cardiomyocytes. CONCLUSION Intracellular calcium triggers PTEN expression.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2006-02-09.基金项目：国家自然科学基金项目资助（No：30370584）通讯作者：祝善俊，教授，主要从事心肌重构方面的研究 Email:19321224@mail.tmmu.com.cn 作者简介：周裔忠,主治医师,博士 Email:zhzh700@yahoo.com.cn
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