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|本期目录/Table of Contents|

胆固醇天然抗体的检测与分析(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第2期
页码:
173-175
栏目:
基础研究
出版日期:
2007-04-01

文章信息/Info

Title:
Analysis of binding capacity of natural antibody to cholesterol
作者:
冯旭阳1;  李巍2; 王海昌1;  付萌2; 邢影2
第四军医大学西京医院：1.心脏内科，2.皮肤科，陕西 西安 710032
Author(s):
FENG Xuyang1;  LI Wei2; WANG Haichang1;  FU Meng2;  XING Ying2
1.Department of Cardiology, 2.Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi′an 710032, Shaanxi, China
关键词:
胆固醇; 天然抗体; 动脉粥样硬化
Keywords:
cholesterol; natural antibody; atherosclerosis
分类号:
R329.12
DOI:
-
文献标识码:
A
摘要:
目的 检测不同年龄正常人血清IgM抗体以及单克隆天然抗体2H8、3B4、4G2对胆固醇的结合。方法 应用间接ELISA法检测新生儿(脐带血)、幼儿、成年人及老年人血清IgM对胆固醇的反应性及其滴度，ELISA和流式细胞仪分析单克隆天然抗体对胆固醇的结合。结果 脐带血中即存在与胆固醇反应的IgM抗体，但其滴度明显低于其它3个年龄组，而其他3组的抗体滴度无明显差异；单抗3B4与胆固醇发生较强的反应。结论 正常人血清中天然存在着与胆固醇发生反应的IgM型自身抗体，天然来源的单克隆抗体3B4可以与胆固醇结合，从而为进一步研究天然抗体在脂质代谢中的作用奠定了基础。
Abstract:
AIM To analyze the binding capacity of serum IgM from healthy individuals and monoclonal natural antibodies to cholesterol. METHODS Levels of IgM reactive with cholesterol in healthy human sera at different ages (newborn babies, infants, adults and the aged) were detected by indirect ELISA and the binding of monoclonal natural antibody to cholesterol was detected by ELISA and FACS. RESULTS Cholesterol reactive IgM was found in the sera of cord blood and the levels of IgM in cord blood were significantly lower than those in the other three groups, but no significant difference was found in the levels in infant, adult and the aged groups. Monoclonal natural antibody 3B4 bound to cholesterol with a strong reactivity. CONCLUSION Cholesterol reactive natural antibodies naturally exist in healthy individuals, and monoclonal antibody 3B4 can bind to cholesterol, which made a foundation for further research on the role of natural antibodies in cholesterol metabolism.

参考文献/References

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［2］ Wick G, Knoflach M, Xu Q. Autoimmune and inflammatory mechanisms in atherosclerosis ［J］. Annu Rev Immunol, 2004, 22: 361-403.

［3］ Binder CJ, Shaw PX, Chang MK, et al. The role of natural antibodies in atherogenesis ［J］. J Lipid Res, 2005, 46(7):1353-1363.

［4］ Horkko S, Bird DA, Miller E, et al. Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipidprotein adducts inhibit macrophage uptake of oxidized lowdensity lipoproteins ［J］. J Clin Invest, 1999, 103(1): 117-128.

［5］ Alving CR, Wassef NM. Naturally occurring antibodies to cholesterol: a new theory of LDL cholesterol metabolism ［J］. Immunol Today, 1999, 20(8): 362-366.

［6］ 付萌，李巍，王刚，等. 天然属性的抗角蛋白单克隆IgM抗体的制备　　［J］. 中华皮肤科杂志, 2005, 38(4): 208-210.

［7］ LacroixDesmazes S，Kaveri SV, Mouthon L, et al. Selfreactive antibodies (natural autoantibodies) in healthy individuals ［J］. J Immunol Methods, 1998, 216(1-2): 117-137.

［8］ Boes M. Role of natural and immune IgM antibodies in immune responses ［J］. Mol Immunol, 2000, 37(18): 1141-1149.

［9］ Freigang S, Horkko S, Miller E, et al. Immunization of LDL receptor-deficient mice with homologous malondialdehyde-modified and native LDL reduces progression of atherosclerosis by mechanisms other than induction of high titers of antibodies to oxidative neoepitopes［J］. Arterioscler Thromb Vasc Biol, 1998, 18(12): 1972-1982.

［10］Shaw PX, Hrkk S, Chang MK, et al. Natural antibodies with the T15 idiotype may act in atherosclerosis, apoptotic clearance, and protective immunity［J］. J Clin Invest， 2000, 105(12): 1731-1740.

［11］李巍, 韩骅, 邢影，等. 高滴度天然抗角蛋白自身抗体转基因小鼠模型的建立 ［J］. 中华皮肤科杂志, 2005, 38(12):759-761.

备注/Memo

备注/Memo:
收稿日期：2006-05-08.基金项目:国家自然科学基金项目资助(No.30300318)作者简介:冯旭阳,主治医生，博士生 Email:fengxuyang@sohu.com
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