短、中期尾吊大鼠动脉局部血管紧张素转化酶与糜酶表达的变化(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2007年第3期
- 页码:
- 277-279/285
- 栏目:
- 基础研究
- 出版日期:
- 2007-06-01
文章信息/Info
- Title:
- Effects of short and midterm tail suspension on expression of ACE and chymase of arteries in rats
- Author(s):
- BAO Junxiang; REN Xinling; MA Jin; BAI Yungang
- 1.Department of Aerospace Physiology, School of Aerospace and Aviation Medicine, 2.Department of Respiratory Disease, Xijing Hospital, Fourth Military Medical University, Xi′an 710032, Shaanxi, China
- 关键词:
- 模拟失重; 大鼠; 肾素－血管紧张素系统; 动脉
- Keywords:
- simulated weightlessness; rats; reninangiotensin system; artery
- 分类号:
- R852.22
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 探讨局部肾素－血管紧张素系统（reninangiotensin system, RAS）在短、中期尾部悬吊致大鼠动脉差异性适应中的作用。方法 采用尾吊1周和4周模拟短期和中期失重影响，取基底动脉和股动脉组织，应用反转录－聚合酶链式反应（RTPCR）检测血管紧张素转化酶（angiotensin converting enzyme, ACE）和糜酶的mRNA的表达水平，并通过脱氧核糖核酸（DNA）序列测定对PCR结果进行验证。结果 与对照组相比，尾吊1周后，基底动脉血管组织ACE的mRNA表达显著增加（P<0.05），糜酶的mRNA表达无明显改变；股动脉血管组织ACE的mRNA表达显著降低（P<0.05），糜酶的mRNA表达无显著改变。悬吊4周后，基底动脉和股动脉血管组织ACE和糜酶的mRNA表达均无显著改变。结论 短期模拟失重可引起ACE在不同动脉血管组织差异性表达的改变。提示局部RAS可能在模拟失重致动脉差异性适应中起重要作用。
- Abstract:
- AIM To explore the role of local reninangiotensin system (RAS) in differential adaptation of arteries to short and midterm simulated weightlessness. METHODS 1 and 4wk tailsuspended rat models were respectively adopted to simulate short and midterm weightlessness. Basilar and femoral arteries were harvested. Reverse transcriptase polymerase chain reaction (RTPCR) was employed to examine the mRNA expression of angiotensin converting enzyme (ACE) and chymase. The PCR products were further confirmed by DNA sequencing. RESULTS After 1wk tail suspension, mRNA expression of ACE in basilar arterial tissue increased significantly (P<0.05), but there were no obvious changes in mRNA expression of chymase. In femoral arterial tissue, mRNA expression of ACE decreased significantly (P<0.05) and mRNA expression of chymase changed insignificantly as compared with that of control level. After 4wk tail suspension, no significant mRNA expression changes of ACE and chymase were observed in both basilar and femoral arterial tissues compared with that in control rats. CONCLUSION Short but not midterm simulated weightlessness may induce differential expression of ACE in different arterial tissues, which indicates local RAS may play a pivotal role in differential adaptation of arteries induced by simulated weightlessness.
参考文献/References
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［2］ Zhang LF, Bao JX. Vascular adaptation to microgravity: role and implications of local vascular reninangiotensin system ［M］//Hargens A, Takeda N, Singal PK. Adaptation Biology and Medicine, Volume 4: current concept. New Delhi: Narosa Publishing House, 2005:329-339.
［3］ Bao JX, Zhang ZT, Zhang LF, et al. Angiotensinogen expression in vascular tissues of simulated weightless rats ［J］. J Gravit Physiol, 2005, 12(l): P83-P84.
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［6］ Dzau VJ. Theodore cooper lecture:Tissue angiotensin and pathobiology of vascular disease: a unifying hypothesis ［J］. Hypertension, 2001, 37(4): 1047-1052.［7］ Miyazaki M, Takai S. Local angiotensin IIgenerating system in vascular tissues: the roles of chymase ［J］. Hypertens Res, 2001, 24(3): 189-193.［8］ Arakawa K, Urata H. Hypothesis regarding the pathophysiological role of alternative pathways of angiotensin II formation in atherosclerosis ［J］. Hypertension, 2000, 36(4): 638-641.
备注/Memo
- 备注/Memo:
- 收稿日期: 2007-02-12.基金项目：国家自然科学基金项目资助（No. 30171032）通讯作者：马进，副教授，主要从事心血管生理学研究Email: jinma@fmmu.edu.cn作者简介：暴军香，讲师，博士Email: baojunx@fmmu.edu.cn
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