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血管紧张素Ⅱ对心脏成纤维细胞PTEN基因表达及增殖的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第4期
页码:
377-379
栏目:
基础研究
出版日期:
2007-08-01

文章信息/Info

Title:
Effect of AngⅡ expression of PTEN gene and proliferation of cardiac fibroblast cells in neonate rats
作者:
聂凌; 王江;  宋熔; 祝善俊
第三军医大学新桥医院心血管内科，重庆 400037
Author(s):
NIE Ling;  WANG Jiang;  SONG Rong;  ZHU Shanjun
Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
心脏成纤维细胞;  血管紧张素II; 第10号染色体同源丢失性磷酸酶张力蛋白基因; 转化生长因子β1; 增殖
Keywords:
cardiac fibroblast cells;  angiotensin Ⅱ; PTEN;  TGFβ1; proliferation
分类号:
R541.6；R394.33
DOI:
-
文献标识码:
A
摘要:
目的 以AngⅡ刺激乳鼠心脏成纤维细胞（CFC），观察CFC的增殖情况以及第10号染色体同源丢失性磷酸酶张力蛋白基因（PTEN）及转化生长因子β1（TGFβ1）基因表达。方法 以AngⅡ（1×10-7mmol/L）刺激分离培养的CFC，以MTT法检测成纤维细胞的增殖情况，以RTPCR的方法检测PTEN及TGFβ1基因表达。结果 MTT测定的结果：32 h后 AngⅡ刺激组的吸光值（A）显著高于该时间点对照组（P<0.05），与正常对照组相比，AngⅡ作用组PTEN基因表达显著下降（P<0.05），TGFβ1基因显著上调（P<0.05）。结论 在AngⅡ的刺激下，CFC 的TGFβ1表达上调，抑制PTEN基因表达，并引起细胞增殖。
Abstract:
AIM To observe the effect of proliferation of cardiac fibroblast cells and expression of PTEN and TGFβ1 gene stimulated by AngⅡ in neonate rats. METHODS Cardiac fibroblast cells (CFCs) were cultured and stimulated by AngⅡ（1×10-7mmol/L）. Cell proliferation was measured by MTT and expressions of PTEN and TGFβ1 gene were measured by RTPCR. RESULTS MTT absorbance (A) of AngⅡstimulated fibroblast cells was significantly higher than that of the control group（P<0.05）after 32 hours. Expression of PTEN increased and expression of TGFβ1 gene decreased compared with those in the control group（P<0.05）. CONCLUSION When CFCs in neonate rats are stimulated by AngⅡ, they upregulate the expression of TGFβ1 gene and downregulate the expression of PTEN gene, thus promoting the proliferation of the cells.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2006-12-06.基金项目：国家自然科学基金项目资助（No.30370584 ）通讯作者：祝善俊，教授，主要从事慢性心力衰竭与心肌重构研究Email: sjzhu@mail.xqhospital.com.cn 作者简介：聂凌，住院医师，博士生 Email:nili@mail.tmmu.com.cn
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