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肿瘤坏死因子α mRNA在小鼠心肌炎中的表达及黄芪治疗对其的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第4期
页码:
413-415/420
栏目:
基础研究
出版日期:
2007-08-01

文章信息/Info

Title:
Effect of Astragalus Membranaceus on myocardial TNFα mRNA in mice with viral myocarditis
作者:
吴凡; 　邱汉婴; 　谢家斌; 　董继华; 　廖玉华
汕头大学医学院临床技能中心，广东 汕头 515031
Author(s):
WU Fan;  QIU Hanying;  XIE Jiabin;  DONG Jihua;  LIAO Yuhua
Clinical Skills Training Center, Medical College, Shantou University, Shantou 515031, Guangdong,China
关键词:
柯萨奇病毒B组; 心肌炎; 肿瘤坏死因子α
Keywords:
Coxsackie virus B;  viral myocarditis;  tumor necrosis factorα
分类号:
R542.21
DOI:
-
文献标识码:
A
摘要:
目的 探讨肿瘤坏死因子α（TNFα）mRNA在柯萨奇病毒B3 （Coxsackie virus B3,CVB3）病毒性心肌炎（viral myocarditis, VMC）小鼠心脏组织中的表情况，应用黄芪治疗后观察TNFα mRNA表达的变化。方法 4周龄纯种雄性BALB/c小鼠86只，随机分成4组。模型组40只，每只腹腔接种100 TCID50的以Hanks液稀释的CVB3m病毒液0.2 ml，分别于接种后1,3,7,9,11,21 d，随机取存活小鼠4只，将其处死，取心脏组织，冻存于-70℃冰箱中，待做RTPCR；黄芪用药组20只，同模型组接种病毒，0.5 h后开始以黄芪口服液给小鼠灌胃，每次0.2 ml， 2次/d，共10 d；未用药组20只，同模型组接种病毒，0.5 h后开始以生理盐水灌胃，每次0.2 ml, 2次/d，共10 d；正常对照组6只，每只腹腔接种Hanks液0.2 ml。于11 d处死用药组、未用药组及正常对照组组所有存活小鼠，取心脏组织冻存于-70℃冰箱中，待作RTPCR。采用半定量RTPCR检测心脏组织中TNFα mRNA的表达情况。结果 ①小鼠病毒性心肌炎心脏组织中TNFα mRNA于3 d表达开始增加，9 d达高峰，随后表达下降，21 d仍可见表达;②给予黄芪治疗可降低心脏组织TNFα mRNA表达水平（P<0.05）。结论 CVB3病毒性心肌炎小鼠心脏组织中TNFα mRNA早期即开始表达，并且其表达随时间而变化；黄芪可通过降低心脏组织中TNFα的表达而发挥其治疗VMC的有益的免疫调节作用。
Abstract:
AIM To investigate the effect of Astragalus Membranaceus (AM) on myocardial TNFα mRNA in mice with Coxsackie virus B3 viral myocarditis. METHODS Eightysix 4weekold male mice were randomly divided into 4 groups of A, B, C and D. Forty mice of group A were inoculated intraperitoneally with 0.2 ml CVB3 diluted in Hanks medium. At day 1, 3, 7, 9, 11 and 21 after virus inoculation, every 4 survived mice were killed, their heart tissues were taken out and stored at 70℃. Twenty mice of group B were perfused with AM to stomach after inoculated with CVB3 for 0.5 h, in a doze of 0.2ml and twice daily for 10 consecutive days. Twenty mice of group C were perfused with saline to stomach in the same dose as group B after inoculated with CVB3 for 0.5h. Six mice of group D as the control were inoculated intraperitoneally only with 0.2 ml Hanks medium. At day 11, all the survived mice were killed and their heart tissues were stored at -70℃. Semiquantitative RTPCR was then used to detect the expression of TNFα mRNA in the heart tissues. RERULTS ① TNFα mRNA increased 3 days after virus inoculation when compared to that of the normal control. The expression peaked at 9 days after inoculation, then decreased and could be seen 21 days after inoculation. ② Treatment with AM decreased the expression of myocardial TNFα mRNA when compared that treated with saline control (P<0.05). CONCLUSIONS The expression of TNFα mRNA increased in mice with CVB3 viral myocarditis and treatment with AM decreases myocardial TNFα mRNA expression.

参考文献/References

［1］ 吴凡，谢家斌，邱汉婴.细胞因子治疗病毒性心肌炎研究进展［J］.心脏杂志，2004,16(2):163-164,168.

［2］ 杨英珍，王齐冰.对病毒性心肌炎诊治的一些见解［J］.中华心血管病杂志，2002，30（3）:129130.

［3］ Seko Y,Takahashi N,Yagita H,et al.Expression of cytokine mRNA in murine hearts with acute myocarditis caused by coxsackievirus b3［J］.J Pathol,1997,183(1):105-108.

［4］ 杨英珍，熊丁丁.病毒性心肌炎治疗现状［J］.中国新药与临床杂志，1998，17（5）：307-309.

备注/Memo

备注/Memo:
收稿日期：2006-09-28.作者简介：吴凡，讲师，硕士Email:wufan746@126.com
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