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|本期目录/Table of Contents|

老年大鼠主动脉对胰岛素敏感性的改变及其机制(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第4期
页码:
409-412
栏目:
基础研究
出版日期:
2007-08-01

文章信息/Info

Title:
Aging effect of insulin sensitivity in rat aorta and its mechanism
作者:
黄洁1; 王海昌1; 马恒2; 高峰2
第四军医大学：1. 西京医院心脏内科，2. 基础部生理学教研室，陕西 西安 710032
Author(s):
HUANG Jie1;  WANG Haichang1;  MA Heng2;  GAO Feng2
1.Department of Cardiology, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi′an 710032, Shaanxi, China
关键词:
增龄改变; 胰岛素敏感性; 一氧化氮; 一氧化氮合酶
Keywords:
aging effect;  insulin sensitivity;  nitric oxide;  endothelial nitric oxide synthase(eNOS)
分类号:
R339.3; R331.3
DOI:
-
文献标识码:
A
摘要:
目的 观察大鼠主动脉对胰岛素敏感性的增龄改变并分析其可能机制。方法 老年组采用老年（18月龄）SD大鼠20只，随机选取成年（15周龄）SD大鼠20只为对照组。采用离体血管灌流技术，观察胸主动脉对胰岛素反应性的变化，并同时测定两组主动脉血管一氧化氮（NO）释放量及血管一氧化氮合酶（eNOS）活性；免疫组织化学法及Western Blot法检测老年组及成年组胸主动脉eNOS的蛋白表达变化。结果 胰岛素可以浓度依赖性舒张成年大鼠胸主动脉，舒血管作用具有内皮依赖性。与之相比，老年大鼠主动脉对胰岛素的舒张反应显著下降(P<0.05)。同时发现，与成年组相比，老年组NO释放量以及eNOS活性显著降低(P<0.05)，免疫组织化学染色显示老年组主动脉eNOS的蛋白表达显著降低；而Western Blot检测发现老年组血管eNOS磷酸化水平显著降低(P<0.05)。结论 血管组织内源性eNOSNO系统活性下降可能是衰老导致的血管胰岛素敏感性下降的重要机制。
Abstract:
AIM To survey the aging effect of insulin sensitivity in rat aorta and to investigate its mechanism. METHODS Twenty aged SD rats (18 months old) and 20 adult SD rats (15 weeks old) as control were studied. The effect of insulin on aorta was assessed using isolated aortic ring preparation and then the tissue concentration of NO, the bioactivity of eNOS and the expression of eNOS protein in both groups were assessed respectively. RESULTS Insulin relaxed adult rat aorta in a dosedependent manner and it was endotheliumdependent, while the vasodilatation of aged aorta to insulin significantly decreased (P<0.05). The aortic NO concentration of the aged reduced compared with that of the adults, so did the bioactivity of eNOS (P<0.05). Immunostaining for eNOS in the aortic endothelium significantly decreased and Western blot showed that the expression of eNOS phospholinase protein also decreased (P<0.05). CONCLUSION The decline insulin sensitivity of aged aorta is related to the weakened eNOSNO activity.

参考文献/References

［1］ Taddei S, Virdis A, Ghiadoni L, et al. Endothelium, aging, and hypertension［J］. Curr Hypertens Rep, 2006, 8(1): 84-89.

［2］ Baron AD, Clark MG. Role of blood flow in the regulation of muscle glucose uptake［J］. Annu Rev Nutr, 1997,17:487- 499.

［3］ Taddei S, Virdis A, Mattei P, et al. Effect of insulin on acetylcholineinduced vasodilation in normotensive subjects and patients with essential hypertension ［J］. Circulation, 1995, 92(10): 2911-2918.

［4］ Cersosimo E, Defronzo RA. Insulin resistance and endothelial dysfunction: the road map to ca rdiovascular diseases［J］. Diabetes Metab Res Rev, 2006, 22(6): 423-436.

［5］ Marcovecchio ML, Patricelli L, Zito M, et al. Ambulatory blood pressure monitoring in obese children: role of insulin resistance［J］. J Hypertens, 2006, 24(12): 2431-2436.

［6］ Unno Y, Akuta T, Sakamoto Y, et al. Nitric oxideinduced downregulation of leptin production by 3T3L1 adipocytes［J］. Nitric Oxide, 2006, 15(2): 125-132.

［7］ Chang AM, Smith MJ, Galecki AT, et al. Impaired betacell function in human aging: response to nicotinic acidinduced insulin resistance［J］. J Clin Endocrinol Metab, 2006, 91(9): 3303-3309.

［8］ Gao F, Christopher TA, Lopez BL, et al. Mechanism of decreased adenosine protection in repe rfusion injury of aging rats［J］. Am J Physiol Heart Circ Physiol, 2000, 279(1):H329-H338.

［9］ Higashi Y, Oshima T, Sasaki N, et al. Relationship between insulin resistance and endothelium dependent vascular relaxation in patients with essential hypertention［J］. Hypertension, 1997, 29(1 Pt 2): 280-285.

［10］Wang CC, Goalstone ML, Draznin B, et al. Molecular mechanisms of insulin resistance that impact cardiovascular biology［J］. Diabetes, 2004, 53(11): 2735-2740.

［11］Bredt DS, Snyder SH. Nitric oxide:a physiologic messenger molecular［J］. Annu Rev Biochem, 1994, 63: 175-195.

［12］Calver A, Collier J, Vallance P, et al. Forearm blood flow responses to a nitric oxide synthase inhibitor in patients with treated essential hypertension［J］. Cadiovasc Res, 1994, 28 (11):1720-1725.

［13］Dananberg J, Sider RS, Grekin RJ. Sustained hypertension induced by orally administered nit roLarginine［J］. Hypertension, 1993, 21(3): 359-363.

备注/Memo

备注/Memo:
收稿日期：2006-11-15.基金项目：国家自然科学基金项目资助（No.39970302）共同通讯作者：高峰，教授，主要从事缺血心脏保护研究Email:gaofeng@fmmu.edu.cn王海昌，教授，主要从事心律失常研究Email:wanghc@fmmu.edu.cn作者简介：黄洁，医师，硕士Email:huangjie@fmmu.edu.cn
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