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肾上腺髓质素对大鼠血管外膜成纤维细胞迁移的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第5期
页码:
517-519/523
栏目:
基础研究
出版日期:
2007-10-01

文章信息/Info

Title:
Effects of Adrenomedullin on rat vascular adventitial fibroblasts migration
作者:
刘海潮; 马业新
华中科技大学同济医学院附属同济医院,湖北 武汉 430030
Author(s):
LIU Haichao;  MA Yexin
Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
关键词:
肾上腺髓质素;  血管外膜成纤维细胞;  骨桥蛋白; 血管紧张素II
Keywords:
adrenomedullin;  vascular adventitial fibroblasts;  osteopontin;  angiotensin II
分类号:
R543.1
DOI:
-
文献标识码:
A
摘要:
目的 研究肾上腺髓质素(ADM)对血管紧张素II(Ang II)诱导的大鼠胸主动脉外膜成纤维细胞迁移的影响。方法 采用体外培养的大鼠胸主动脉外膜成纤维细胞,运用Transwell技术测定不同浓度ADM对外膜成纤维细胞迁移的影响,用RTPCR及Western blotting分析Ang II (1×10-6 mol/L)及不同浓度ADM干预后大鼠胸主动脉外膜成纤维细胞内骨桥蛋白的表达。结果 在Ang II (1×10-6 mol/L)趋化作用下,外膜成纤维细胞迁移活性较对照组显著增强，ADM可抑制Ang II刺激的细胞迁移,迁移细胞数目在一定范围内随着ADM浓度增加而减少；Ang II (1×10-6 mol/L)诱导骨桥蛋白呈高表达, ADM可下调这种表达,呈一定剂量依赖性。结论 ADM明显抑制Ang II刺激的外膜成纤维细胞迁移, 并且ADM可以抑制Ang II刺激的骨桥蛋白表达。
Abstract:
AIM To explore the effect of Adrenomedullin on angiotensin IIinduced migration in cultured rat vascular adventitial fibroblasts. METHODS Rat vascular adventitial fibroblasts were cultured in vitro and the effect of Adrenomedullin on the migration of adventitial fibroblasts was evaluated by transwell. The expression of osteopontin in adventitial fibroblasts stimulated in different conditions was measured by RTPCR and Western blotting. RESULTS Ang II stimulated adventitial fibroblasts migration and Adrenomedullin clearly inhibited Ang IIinduced migration of adventitial fibroblasts in a dosedependent manner between 1×10-9 and 1×10-7 mol/L. Ang II stimulated osteopontin expression in these cells and the inhibition by Adrenomedullin of Ang IIinduced adventitial fibroblasts migration was paralleled by a decrease in the expression of osteopontin. CONCLUSION ADM can inhibit adventitial fibroblasts migration and osteopontin expression induced by Ang II.

参考文献/References

［1］ Yoshimoto T,Fukai N,Sato R,et al. Antioxidant effect of adrenomedullin on angiotensin IIinduced reactive oxygen species generation in vascular smooth muscle cells ［J］. Endocrinology，2004,145(7):3331-3337.

［2］ Shi Y,O’Brien JE,Fard A,et al. Adventitial myofibroblasts contribute to neointimal formation in injured porcine coronary arteries ［J］. Circulation,1996,94（7）:1655-1664.

［3］ Yang JH, Jiang W, Pan CS, et al. Effects of adrenomedullin on cell proliferation in rat adventitia induced by lysophosphatidic acid ［J］. Regul Pept，2004,121(1-3):49-56.

［4］ Jiang W, Yang JH, Pan CS, et al. Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone ［J］. J Hypertens，2004,22(10):1953-1961.

［5］ Tsuruda T, Kato J, Cao YN, et al. Adrenomedullin induces matrix metalloproteinase2 activity in rat aortic adventitial fibroblasts ［J］. Biochem Biophys Res Commun，004,325(1):80-84.

［6］Tsuruda T, Kato J, Matsui E, et al. Adrenomedullin alleviates not only neointimal formation but also perivascular hyperplasia following arterial injury in rats ［J］. Eur J Pharmacol，2005,508(1-3):201-204.

［7］ 孙爱军,高平进,朱鼎良,等.骨桥蛋白在血管外膜中的表达及其对外膜成纤维细胞迁移的影响［J］. 中华心血管病杂志，2003， 31（5）： 361-364.

备注/Memo

备注/Memo:
收稿日期：2006-08-01.作者简介:刘海潮,博士生Email:lhc197628@163.com
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