我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

咖啡酸苯乙酯对激活的大鼠血管平滑肌细胞增殖的抑制作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第5期
页码:
513-516
栏目:
基础研究
出版日期:
2007-10-01

文章信息/Info

Title:
Inhibitive effects of caffeic acid phenethyl ester on proliferation of rat vascular smooth muscle cells activated by lipopolysaccharide
作者:
杨刚;  常超;  王裕勤;  冯义柏;  荣书玲
华中科技大学同济医学院附属协和医院心研所、心内科， 湖北 武汉 430022
Author(s):
YANG Gang;  CHANG Chao;  WANG Yuqin;  FENG Yibo;  RONG Shuling
Research Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China
关键词:
咖啡酸苯乙酯; 血管平滑肌; 增殖; 存活素; 增殖细胞核抗原
Keywords:
caffeic acid phenethyl ester;  vascular smooth muscle cells;  proliferation;  Survivin;  proliferating cell nuclear antigen
分类号:
R543
DOI:
-
文献标识码:
A
摘要:
目的 探讨脂多糖（lipopolysaccharide, LPS）激活血管平滑肌细胞(vascular smooth muscle cells, VSMC)后，咖啡酸苯乙酯（caffeic acid phenethyl ester, CAPE）对细胞过度增殖的影响。方法 采用不同浓度的CAPE处理LPS激活的VSMC。MTT法用于检测细胞的生长情况；免疫细胞化学法检测增殖核抗原（PCNA）和存活素（Survivin）蛋白的阳性率；流式细胞仪检测细胞周期分布。结果 5、10、20、40、80 mg/L CAPE 处理LPS激活的VSMC后，MTT检测发现细胞的生长明显受到抑制并呈剂量和时间依赖；VSMC经CAPE处理72 h后, 细胞中 PCNA和Survivin蛋白阳性表达率随药物剂量增加而显著降低（P<0.05）；细胞周期分析表明5、10、20 mg/L CAPE处理VSMC 24 h后，对照组和实验组G0/G1期细胞百分率分别为(40±4.3)％和(52±6)％，(65±1)％，(76±4)％，S期细胞百分率由对照组的(32.4%±2.6)%逐渐下降20 mg/L组的(11.2%±1.4)％，呈剂量依赖性（P<0.05）。结论 CAPE可能通过调控细胞周期，减少PCNA和Survivin蛋白的表达而发挥细胞增殖抑制作用，其效能与作用时间和药物剂量存在一定的相关性。
Abstract:
AIM To investigate the effects of caffeic acid phenethyl ester (CAPE) on the proliferation of vascular smooth muscle cells (VSMC)activated by lipopolysaccharide (LPS). METHODS VSMC were activated by 1 mg/L LPS and the effect of CAPE on the proliferation of the cells was defected by using methabenzthiazuron (MTT) assay. The cells positive for proliferating cell nuclear antigen (PCNA) and Survivin protein were measured with immunocytochemistry staining method. Cell cycles were analyzed by using flow cytometry (FCM) with propidium iodide (PI) labeling method. RESULTS CAPE exerted a significant inhibitory effect on the proliferation of VSMC induced by 1 mg/L LPS at concentrations ranging from 5 mg/L to 80 mg/L and significantly repressed the expression of PCNA and Survivin protein in a dose and timedependent manner（P<0.05）. After cells had been exposed to CAPE (5,10,20 mg/L) for 24 h, FCM analysis showed that the rates of cells in G0/G1 stage were correspondingly 40.1％±4.3％ in the control and 52.3％±5.5％, 64.5％±1.3％, 76.2％±3.7％ in the treated groups, and the percentage of S stage decreased from 32.4%±2.6% of the control to 11.2%±1.4％ of the 20 mg/L group（P<0.05）. CONCLUSION CAPE significantly inhibits the proliferation of VSMC activated by LPS through regulating cell cycles of VSMC and inhibiting the expression of PCNA and Survivin protein in a dose and timedependent manner.

参考文献/References

［1］ Usia T, Banskota AH, Tezuka Y,et al. Constituents of Chinese propolis and their antiproliferative activities ［J］. J Nat Prod, 2002, 65 (5): 673-676.

［2］ Chen YJ, Shiao MS, Wang SY. The antioxidant caffeic acid phenethyl ester induces apoptosis associated with selective scavenging of hydrogen peroxide in human leukemic HL60 cells［J］. Anticancer Drugs, 2001, 12 (2):143-149.

［3］ 李剑，林树新，李莹，等. 脂多糖对离体培养大鼠血管平滑肌细胞收缩的影响［J］. 第四军医大学学报,1999，20 (7）：610-612.

［4］ 向德兵,何渝军,吴晓华，等. 咖啡酸苯乙酯对大肠癌HCT116细胞增生的抑制作用［J］. 世界华人消化杂志, 2004，12(5):1053-1056.

［5］ Prosperi E. Multiple roles of the proliferating cell nuclear antigen: DNA replication, repair and cell cycle control［J］. Prog Cell Cycle Res, 1997, 3:193-210.

［6］ Suzuki A, Ito T, Kawano H, et al. Survivin initiates ptrocapase 3/p21 complex formation as a result of interaction with Cdk4 to resist Fasmediated cell death［J］. Oncogene,2000, 19(10):1346-1353.

［7］ Johnson AL, Langer JS, Bridgham JT. Survivin as a cell cyclerelated and antiapoptotic protein in granulosa cells［J］. Endocrinology,2002, 143(9): 3405-3413.

［8］ Ambrosini G, Adida C, Altieri DC. A novel antiapoptosis gene, Survivin, expressed in cancer and lymphoma［J］. Nat Med, 1997, 3(8):917-921.

［9］ Ai MD, Li LL, Zhao XR,et al. Regulation of Survivin and CDK4 by EpsteinBarr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cell lines［J］. Cell Res,2005, 15 (10):777-784.

备注/Memo

备注/Memo:
收稿日期：2006-06-23.作者简介：杨刚，住院医师，博士Email:yanggang1688@163.com
更新日期/Last Update: