文章摘要

文章信息/Info

Title:: Interventional effect of Losartan on expression of MCP1 and CD11b in experimental cholesterolfed rabbits

作者:: 王夜明¹; 曾秋棠² ; 袁杰²; 1. 湖北省新华医院心内科,湖北武汉 430015;2. 华中科技大学同济医学院协和医院心血管研究所，湖北武汉 430015

Author(s):: WANG Yeming¹; ZENG Qiutang²; YUAN Jie²; 1.Department of Cardiology, Hubei Xinhua Hospital, Wuhan 430015, Hubei, China; 2.Department of Cardiology, Union Hospital, Tongji Medical College, HuaZhong University of Science and Technology, Wuhan 430015, Hubei, China

关键词:: 动脉粥样硬化; 单核细胞趋动蛋白1; CD11b; 氯沙坦

Keywords:: atherogenesis; MCP1; CD11b; losartan

分类号:: R332;R341.31;R342.22

DOI:: -

文献标识码:: A

摘要:: 目的观察氯沙坦对高胆固醇血症兔主动脉组织单核细胞趋动蛋白1 （MCP1）基因表达水平和静脉血单核细胞表面黏附分子CD11b活性（CD11b）的影响。方法 40只日本长耳白兔随机分为4组，每组10只。正常对照组喂以普通饲料；高胆固醇组喂以高胆固醇饲料；氯沙坦高、低剂量组在喂高胆固醇饲料同时，分别以氯沙坦粉剂25 mg/(kg·d)和10 mg/（kg·d）溶于温水中灌胃，1次/d 。观察12周。实验结束时，主动脉组织经HE染色行病理学检查。用流式细胞技术检测CD11b。用RTPCR方法检测主动脉组织MCP1的基因表达。结果高胆固醇组血管内膜下斑块形成明显，而氯沙坦高、低剂量组斑块明显减少；高胆固醇组较对照组MCP1 mRNA表达及CD11b活性显著增高(P<0.01 )；氯沙坦高、低剂量组MCP1 mRNA表达及CD11b活性较高胆固醇组显著降低(P<0.05)。结论氯沙坦抑制组织MCP1 mRNA的表达及降低CD11b活性，降低单核细胞内膜下浸润，其作用机制可能是氯沙坦从受体水平阻断肾素血管紧张素醛固酮系统。

Abstract:: AIM To investigate the effect of Losartan on the expression of MCP1 mRNA and the activity of CD11b. METHODS Atherosclerotic rabbit models were made by feeding the rabbits with high cholesterol. Forty long ear Japan white rabbits were randomly divided into four groups: Group A were fed with common forage, Group B were fed with high cholesterol diet, while group C and D were fed with high cholesterol diet and simultaneously given Losartan of 25 mg/(kg·d) and 10 mg/(kg·d), respectively. At the end of 12 weeks, flow cytometry analysis was applied to detect CD11b of blood samples from vein by FITC labeled antiCD11b. The arteries were isolated and the samples adjacent to aortic arch were harvested for microscopy detection. The MCP1 gene expressions in rabbit thoracic aorta were detected by RTPCR. RESULTS The subintimal plaque of group B significantly increased, but the subintimal plaque of group C and D decreased significantly. Compared with those in group A, the mRNA expression of MCP1 and CD11b values in group B significantly increased（P<0.01） while those in group C and group D significantly decreased when compared with those in group B（P<0.05）. There was no significant difference between group C and D. CONCLUSION Losartan retards atherogenesis by suppressing MCP1 gene expression in tissues and deactivating circulational monocytes. The possible mechanism behind this may be that Losartan blocks RAS at the receptor level.

参考文献/References

［1］ Kato M, Sada T, Mizuno M, et al. Effect of combined treatment with an angiotensin II receptor antagonist and an HMGCoA reductase inhibitor on atherosclerosis in genetically hyperlipidemic rabbits［J］. J Cardiovasc Parmacol, 2005，46(4):556-562.

［2］ Prasad A, Koh KK, Schenke WH, et al. Role of angiotensin II type 1 receptor in the regulation of cellular adhesion molecules in atherosclerosis［J］ . Am Heart J,2001，142(2):248-253.

［3］ Pueyo ME, Gonzalez W, Nicoletti A, et al. Angiotensin Ⅱ stimulates endothelial vascular cell adhesion moleculer1 via nuclear factor kappaB activation induced by intracellular oxidative stress［J］. Arterioscler Thromb Vasc Biol, 2000,20（3）:645-651.

［4 ］孙艺红，黄永麟，张瑞英. 福辛普利对高脂诱发动脉粥样硬化内皮细胞黏附分子1 mRNA 表达的影响［J］.中华心血管病杂志，2001，29（11）：683-684.

［5］徐红新，李庚山，李建军. 氯沙坦对兔动脉粥样硬化消退作用的初步观察［J］.中华心血管病杂志，2002，30（8）：496-499.

［6］秦涛, 李虎, 贾国良,等.卡维地络对心绞痛患者白细胞表面CD11b及CD49d表达的影响［J］.心脏杂志，2003, 15(1)： 37-39.

［7］杨彬,成蓓,刘承云, 等.氯沙坦在损伤血管重构中的作用及其机制的探讨［J］.心脏杂志，2004, 16(1)：23-25,28.

［8］ Oguchi S, Dimayuga P, Zhu J, et al. Monoclonal antibody against vascular cell adhesion molecule1 inhibits neointimal formation after periadventitial carotid artery injury in genetically hypercholesterolemic mice［J］.Arteroscler Thromb Vasc Biol,2000,20(7):1729-1736.

我们的网站为什么显示成这样？

实验性高脂血症兔MCP1与CD11b的表达及氯沙坦的干预作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

文章信息/Info

参考文献/References

备注/Memo

常用功能

导航/Navigate

工具/Tools

统计/Statistics