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甲状腺素对大鼠慢性心衰细胞收缩功能及钙瞬变的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第4期
页码:
410-413
栏目:
基础研究
出版日期:
2008-08-20

文章信息/Info

Title:
Effect of on contract function and calcium transient of chronic heart failure cells in rats
作者:
谢念林1; 俞世强1; 张海锋2;  魏来1; 毕生辉1; 王显悦1
第四军医大学: 1.西京医院心血管外科,2.基础部生理学教研室,陕西 西安 710032
Author(s):
XIE Nianlin1;  YU ShiQiang1;  ZHANG Haifeng2;  WEI Lai1;  BI Shenghui1;  WANG Xian yue1
1.Department of Cardiovascular Surgery, Xijing Hospital, 2.Department of Physiological, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
甲状腺素; 心衰细胞; 动缘探测
Keywords:
thyroid hormone;  heart failure cells;  motion edgedetection
分类号:
R541.6
DOI:
-
文献标识码:
A
摘要:
目的 研究甲状腺素对心力衰竭大鼠的单个心肌细胞收缩功能及钙瞬变的影响。方法 采用腹主动脉缩窄法建立大鼠心力衰竭模型，随机分为心衰组与甲状腺素治疗组，另设假手术对照组。术后16周测定心功能。各组分离单个心室细胞，采用可视化动缘探测系统同步检测大鼠心肌细胞收缩和钙瞬变的变化。结果 ①甲状腺素治疗组左室收缩压（LVSP）和±dp/dtmax较CHF组明显提高（P<005），左室舒张末压（LVEDP）较CHF组明显下降（P<005）。②甲状腺素治疗组单个心肌细胞收缩功能指标最大收缩幅度（PTA）、最大缩短和复长速率（±dL/dtmax）较CHF组明显增加（P<005），钙瞬变指标fura2荧光强度变化（ΔFFI）增强、Ca2+离子达峰值时程（TTPCa）、舒张期Ca2+减少50%时程（T50DCa）较CHF组明显缩短（P<005）。结论 甲状腺素可改善大鼠单个心衰细胞的收缩功能及钙瞬变幅度，在单细胞水平上为探讨甲状腺素治疗慢性心衰的发生机制提供了实验依据。
Abstract:
AIM To investigate the effect of thyroid hormone on contractile function and calcium transient of myocytes isolated from ventricle of rats with chronic heart failure. METHODS A total of 90 SD rats were randomly divided into three groups: control group, heart failure group and thyroid hormone treated group. Chronic heart failure (CHF) was induced by aortic constriction and ventricular myocytes were then enzymatically isolated. Contractile function and calcium transient of the myocytes were detected simultaneously and compared with those in the control group by videobased motion edgedetection system. RESULTS ①Left ventricular systolic pressure (LVSP) and ±dp/dtmax dramatically increased and left ventricular enddiastolic pressure (LVEDP) significantly descended in thyroid hormone treated group compared with those ion the heart failure group (P<001). ②PTA, ±dL/dtmax , ΔFFI, TTPCa and T50DCa of the thyroid hormone treated group were significantly higher than those of the heart failure group. CONCLUSION Thyroid hormone improves the contractile function and calcium transient level of ventricle myocytes isolated from rats with CHF, which may provide an experimental support for the further research on the mechanism of CHF treated with thyroid hormone.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-09-24.通讯作者：俞世强，主任医师，主要从事微创心脏外科、心脏 移植研究Email：yushiq@fmmu.edu.cn 作者简介：谢念林，硕士生Email:maoerer@sina.com
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