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人主动脉瓣间质细胞的分离、培养、鉴定及表型分析

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第2期
页码:
206
栏目:
基础研究
出版日期:
2009-03-30

文章信息/Info

Title:
Isolation, culture, identification and phenotypic analysis of human aortic valve interstitial cells
作者:
江磊; 徐平; 高洪波; 郝传吉; 黄强; 王伟; 于仁斌
青岛大学医学院附属医院心血管外科，山东 青岛 266003
Author(s):
JIANG Lei;  XU Ping;  GAO Hong-bo;  HAO Chuan-ji;  HUANG Qiang;  WANG Wei;  YU Ren-bin
Department of Cardiac Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong, China
关键词:
主动脉瓣; 间质细胞; 肌成纤维细胞; 先天畸形; 风心病
Keywords:
aortic valve;  interstitial cell;  myofibroblast;  congenital malformation;  rheumatic heart disease
分类号:
R322.11
DOI:
-
文献标识码:
A
摘要:
目的 建立人主动脉瓣间质细胞体外培养方法并鉴定其形态、类型及膜表面分子分布。方法 将瓣膜置换术中获取的畸形瓣膜及风心病瓣膜内皮层剥除，用I型胶原酶消化后铺养。光镜下观察细胞的形态，将其以抗人α-平滑肌肌动蛋白抗体及核染色双标记后，以激光共聚焦显微镜观察细胞的类型。以抗干扰素(IFN)-γ受体单抗及抗肿瘤坏死因子(TNF)-α受体单抗标记细胞后，用流式细胞术分析细胞膜分子的分布。 结果 分离培养的主动脉间质细胞主要为肌成纤维细胞。畸形瓣膜和风心病瓣膜表面IFN-γ受体及TNF受体的分布状态不同。结论 成功地建立了体外培养瓣膜间质细胞的方法，并发现来源不同的间质细胞膜表面分子的分布迥异。
Abstract:
AIM To isolate and culture human aortic valve interstitial cells and to identify their morphology, subtype and membrane molecules distribution. METHODS The endothelial cell layers on the dissected valves were removed and the remaining interstitial layers were minced and digested with collagenase I. Cells were then seeded and maintained on culture dishes. Cells were observed under light microscopy and cellular α-smooth muscle actin was detected with confocal staining microscopy. The expressed interferon(IFN)-γ and tumor necrosis factior(TNF)-α receptors were analyzed by flow cytometry with their corresponding monoclonal antibodies. RESULTS The isolated valves interstitial cells were proved to be myofibroblast. More IFN-γ receptors and TNF-α receptors were expressed on rheumatic aortic valves interstitial cells than on non-rheumatic cells and their expression was patients’ sera dependent. CONCLUSION The valves interstitial cells culture system has been established successfully and the membrane molecules differ according to their origins.

参考文献/References

[1］ 戴煌,慈书平. 退行性心脏瓣膜病的心血管并发症［J］. 心脏杂志, 2006, 18(6):727-729.

［2］ 彭万军,朱玲军,王审,等. 退行性心瓣膜病的相关因素［J］. 心脏杂志, 2006, 18(6):742.

［3］ 夏云峰,罗北捷,刘润梅,等. 临床症状及无创检查对老年心肌病和(或)瓣膜病患者并发冠心病诊断真实性的评价［J］. 心脏杂志, 2004, 16(5):488-489.

［4］ Zacks S, Rosenthal A, Granton B, et al. Characterization of cobblestone mitral valve interstitial cells［J］. Arch Pathol Lab Med, 1991, 115(8):774-779.

［5］ Li QY, Jones PL, Lafferty RP, et al. Thymosin beta4 regulation, expression and function in aortic valve interstitial cells［J］. J Heart Valve Dis, 2002, 11(5):726-735.

［6］ Latif N, Sarathchandra P, Taylor PM, et al. Localization and pattern of expression of extracellular matrix components in human heart valves［J］. J Heart Valve Dis, 2005, 14(2):218-227.

［7］ Messier RH Jr, Bass BL, Aly HM, et al. Dual structural and functional phenotypes of the porcine aortic valve interstitial population: characteristics of the leaflet myofibroblast［J］. J Surg Res, 1994, 57(1):1-21.

［8］ Walker GA, Masters KS, Shah DN, et al. Valvular myofibroblast activation by transforming growth factor-beta: implications for pathological extracellular matrix remodeling in heart valve disease［J］. Circ Res, 2004, 95 (3):253-260.

［9］ Guilherme L, Cury P, Demarchi LM, et al. Rheumatic heart disease: proinflammatory cytokines play a role in the progression and maintenance of valvular lesions［J］. Am J Pathol, 2004, 165(5):1583-1591.

［10］Kim-Schulze S, Taback B, Kaufman HL. Cytokine therapy for cancer［J］. Surg Oncol Clin N Am, 2007, 16(4):793-818.

备注/Memo

备注/Memo:
收稿日期：2008-5-26.通讯作者：徐平，主任医师，主要从事先心病及风湿性心脏病研究Email:pingxu688@sohu.com 作者简介：江磊，主治医师，博士Email:qdjianglei@gmail.com
更新日期/Last Update: 2009-04-16